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用高压气相色谱－质量分析法，检测了链脲菌素糖尿病大鼠发病后肾小球内葡葡糖，山梨醇，果糖含量，并同时测量体重，２４小时尿量，２４小时尿蛋白含量、内生肌酐清除率（Ｃｃｒ）及血糖。结果表明，糖尿病大鼠４周组处于临床糖尿病肾病发病前状态，而糖尿病大鼠１２周组已达到临床糖尿病肾病阶段，此两组糖尿病大鼠肾小球内葡萄糖、山梨醇、果糖含量均明显高于各自正常对照组，整个山梨醇途径处于代谢亢进状态。而随着糖尿病肾病的发展，糖尿病大鼠肾小球内在葡萄糖明显增高状态下，而山梨醇含量与糖尿病肾病发病前阶段相比呈下降趋势，果糖含量则无明显变化，提示在糖尿病状态下，肾小球内山梨醇代谢途径的异常变化与糖尿病肾病的发病及进展密切相关。
The content of glucose, sorbitol and fructose in the glomeruli of streptozotocin-induced diabetic rats were detected by high pressure gas chromatography-mass spectrometry. The body weight, 24-hour urine output, 24-hour urine protein content, Endogenous creatinine clearance (Ccr) and blood glucose. The results showed that diabetic rats 4 weeks group is in the pre-morbid state of diabetic nephropathy, and diabetic rats 12 weeks group has reached the stage of clinical diabetic nephropathy, the two groups of diabetic rats glomerular glucose, sorbitol, fructose were significantly Higher than the normal control group, the sorbitol pathway is hypermetabolic. With the development of diabetic nephropathy, glomerular glucose in diabetic rats significantly increased state, while the sorbitol content and diabetic nephropathy compared to the pre-stage stage of decline, no significant changes in fructose content, suggesting that in the diabetic state, Abnormal changes of glomerular intracellular sorbitol metabolism are closely related to the pathogenesis and progression of diabetic nephropathy.