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目的:研制可诱导的小鼠狼疮样肾炎模型。方法:本研究利用正常成年DBA/2J与C57BL/6杂交子一代(B6D2F1)(雄性)小鼠为受体鼠,接受多次大量DBA/2J(雄性)小鼠脾脏、胸腺、淋巴结细胞的静脉输注(每周2次,共4次),2个月左右大部分受体小鼠出现局部胡须脱落、腹水、毛发变色等现象。结果:ELISA显示血清中IgG、IgG1和IgG2含量明显增加(P<0.01)。考马斯亮蓝染色法检测显示,近80%实验组小鼠出现蛋白尿,而对照组小鼠未发现存在尿蛋白。病理学分析显示,皮肤、肠道、肝脏和肾脏均有炎性细胞浸润,尤以肝脏、肾脏病变为著。结论:提示已初步建立了较稳定的小鼠狼疮样肾炎模型,为相关后续研究提供了有益的资料。
Objective: To develop an inducible mouse model of lupus nephritis. Methods: Normal adult DBA / 2J and C57BL / 6 hybrid generation (B6D2F1) (male) mice were used as recipients and received multiple intravenous administrations of splenic, thymus and lymph node cells in DBA / 2J (male) Infusion (2 times a week, a total of 4 times), 2 months or so most of the recipient mice appeared beard off, ascites, hair discoloration and so on. Results: ELISA showed that serum IgG, IgG1 and IgG2 levels were significantly increased (P <0.01). Coomassie brilliant blue staining test showed that nearly 80% of mice in the experimental group showed proteinuria, while the control group mice did not find the presence of urinary protein. Pathological analysis showed that the skin, intestine, liver and kidney have inflammatory cell infiltration, especially liver and kidney lesions. Conclusion: This study suggests that a stable model of lupus-like nephritis in mice has been preliminarily established, which provides useful information for subsequent studies.