论文部分内容阅读
本文旨在研究染料木素(genistein)对去卵巢大鼠基底前脑胆碱能神经元的影响。雌性大鼠双侧卵巢切除2周后用genistein和雌激素替代治疗1周。称子宫重量以确定手术是否成功及雌二醇(E2)的治疗是否有效。用免疫组化染色、RT-PCR和Westernblot等方法对胆碱能神经元数量、ChAT基因和蛋白的表达量进行检测。结果显示:去卵巢3周后子宫变轻,雌激素替代治疗能增加去卵巢子宫的重量,而genistein替代治疗对去卵巢子宫的重量影响不明显;去卵巢3周后,内侧隔核(MS)和斜角带垂直臂核(VDB)内的胆碱能神经元数量、ChAT基因和蛋白的表达量均明显减少,雌激素和genistein替代治疗后能显著增加去卵巢大鼠MS和VDB内的胆碱能神经元数量、ChAT基因和蛋白的表达量。本研究结果提示:genistein对去卵巢大鼠基底前脑胆碱能神经元具有类似雌激素样神经保护作用,而对子宫影响不明显。
This article aims to investigate the effect of genistein on basal forebrain cholinergic neurons in ovariectomized rats. Two weeks after bilateral ovariectomy, female rats were treated with genistein and estrogen replacement for 1 week. Weigh the uterus to determine whether the surgery was successful and whether estradiol (E2) treatment was effective. The number of cholinergic neurons, ChAT gene and protein expression were detected by immunohistochemical staining, RT-PCR and Western blot. The results showed that the uterus became lighter after 3 weeks of ovariectomy, and estrogen replacement therapy could increase the weight of the ovariectomized uterus, while genistein replacement therapy had no significant effect on the weight of ovariectomized uterus; after 3 weeks of ovariectomy, the medial septum (MS) The number of cholinergic neurons, the expression of ChAT genes and proteins in the VDB were significantly reduced, and replacement of estrogen and genistein significantly increased the levels of cholinergic neurons in MS and VDB of ovariectomized rats. Alkalinergic neurons, expression of ChAT genes and proteins. The results of this study suggest that genistein has similar estrogen-like neuroprotective effects on basal forebrain cholinergic neurons in ovariectomized rats, but has no significant effect on the uterus.