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目的探讨自发性高血压大鼠(SHR)局灶性脑缺血/再灌注模型制备方法及其可行性。方法采用线栓法对84只雄性SHR进行局灶性脑缺血/再灌注模型制作,并以30只SD大鼠作为对照。术后24h应用神经行为学评分、氯化三苯基四氮唑(TTC)染色法评价大鼠局灶性脑缺血/再灌注模型制备的成功率及可靠性。结果SHR组模型制作成功60只(71.4%),术中、术后死亡20只(23.8%)、未成功4只(4.8%);而SD大鼠模型制作成功26只(86.7%),术中、术后死亡3只(10.0%),未成功1只(3.3%)。SHR组模型制作成功率低于SD大鼠,但两者比较,差异无统计学意义(χ2=3.69,P>0.05)。SHR组脑梗死面积百分比平均值较SD大鼠组显著增加[(42.6±5.6)%比(29.5±6.7)%,P<0.05],SHR组神经行为学损伤亦更严重(P<0.05)。结论采用线栓法制作SHR局灶性脑缺血/再灌注模型方法可行,稳定性较好。
Objective To investigate the feasibility and feasibility of focal cerebral ischemia / reperfusion model in spontaneously hypertensive rats (SHR). Methods 84 male SHRs were subjected to focal cerebral ischemia-reperfusion model by thread-plug method. Thirty SD rats were used as control. Neurobehavioral score and triphenyltetrazolium chloride (TTC) staining were used to assess the success rate and reliability of focal cerebral ischemia / reperfusion model preparation 24h after operation. Results Sixty (71.4%) models were successfully established in SHR group, 20 (23.8%) died and 4 (4.8%) failed after operation, while 26 rats (86.7%) were successful in SD rat model Among them, 3 died (10.0%) and 1 failed (3.3%). The successful rate of SHR model was lower than that of SD rats, but the difference was not statistically significant (χ2 = 3.69, P> 0.05). The percentage of cerebral infarction area in SHR group was significantly higher than that in SD group [(42.6 ± 5.6)% vs (29.5 ± 6.7)%, P <0.05]. The neurobehavioral injury was also more serious in SHR group (P <0.05). Conclusion The method of making the focal cerebral ischemia / reperfusion model by SHR method is feasible and the stability is good.