目的:建立紫外分光光度法测定广西不同产地倒地铃全草总黄酮的含量。方法:以芦丁为对照品,以亚硝酸钠-硝酸铝-氢氧化钠为显色剂,测定波长为488 nm。结果:芦丁在0.009 35～0.056 0 g·L-1与峰面积具有良好的线性关系(r=0.999 3);平均加样回收率为100.05%(n=6),RSD 2.3%。结论:该法准确、简便、稳定可靠,可用于倒地铃中总黄酮的含量测定。 Objective: To establish a UV spectrophotometric method for the determination of total flavonoids in Rhizoma Polygonatum from different areas in Guangxi. Methods: Rutin was used as reference substance and sodium nitrite - aluminum nitrate - sodium hydroxide as chromogenic reagent. The wavelength of determination was 488 nm. Results: The linear range of rutin was 0.009 35 ~ 0.056 0 g · L-1 with the peak area (r = 0.999 3). The average recoveries were 100.05% (n = 6) and 2.3% RSD. Conclusion: The method is accurate, simple, stable and reliable, and can be used for determination of total flavonoids in Rhizoma.