论文部分内容阅读
目的探讨日本血吸虫抗独特型抗体NP30对急性血吸虫病的免疫治疗作用。方法根据L9(34)正交表设计动物实验,日本血吸虫尾蚴感染C57BL/6小鼠后注射抗独特型抗体NP30,观察不同治疗方案各组小鼠的存活时间并计算不同时间段的生存率,取小鼠肝脏石蜡切片,HE染色,测量小鼠单个虫卵肉芽肿的直径及面积。结果各实验组小鼠的生存中位数为45～78d,其中第4组生存中位数为71d,比同样感染40条尾蚴的对照组2(53d)延长了33.96%。Cox回归分析显示小鼠存活时间主要与尾蚴感染水平、抗体注射途径有关(P均<0.05)。各实验组小鼠单个虫卵肉芽肿平均直径为179.07～226.86μm,平均面积为(32.11～51.37)×103μm2;对照组各组小鼠单个虫卵肉芽肿平均直径为205.89～239.86μm,平均面积为(44.61～57.24)×103μm2。极差分析及方差分析均显示抗独特型抗体NP30的注射方式和注射剂量是影响虫卵肉芽肿平均直径和面积的主效应因素。NP30的最佳免疫治疗方案为感染尾蚴28d后,以每鼠每只20μg的剂量连续3次肌肉注射。结论抗独特型抗体NP30可改善血吸虫感染小鼠的生存状况,降低血吸虫对宿主造成的免疫病理损害,具有治疗急性血吸虫病的潜能。
Objective To investigate the immunotherapeutic effect of Schistosoma japonicum anti-idiotypic antibody NP30 on acute schistosomiasis. Methods According to the L9 (34) orthogonal test, the anti-idiotypic antibody NP30 was injected into C57BL / 6 mice infected with cercariae of Schistosoma japonicum. The survival time of mice in each treatment group was observed and the survival rates of different groups were calculated. Mouse liver paraffin sections were taken and stained with HE to measure the diameter and area of single mouse egg granuloma. Results The median survival of mice in each experimental group was 45-78 days. The median survival time in group 4 was 71 days, which was 33.96% longer than that of control group 2 (53 days) infected with 40 cercariae. Cox regression analysis showed that the survival time of mice was mainly related to cercariae infection and antibody injection (P <0.05). The average diameter of a single egg granuloma in each experimental group was 179.07 ~ 226.86μm and the average area was (32.11 ~ 51.37) × 103μm2. The average diameter of single egg granuloma in each group was 205.89-239.86μm, the average area (44.61 ~ 57.24) × 103μm2. Both range analysis and ANOVA showed that injection of anti-idiotypic antibody NP30 and injection dose were the main effect factors affecting the average diameter and area of ova granuloma. NP30 best immunotherapy program for cercariae 28d after infection, each mouse per 20μg dose of 3 consecutive intramuscular injections. Conclusion Anti-idiotypic antibody NP30 can improve the survival of mice infected with Schistosoma japonicum and reduce the immunopathological damage caused by Schistosoma japonicum to the host, which has the potential of treating acute schistosomiasis.