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目的:研究贝母地上部位提取物的提取纯化工艺,确定工艺参数。方法:以总生物碱为考察指标,采用酸性染料比色法测定总生物碱含量,对影响总生物碱提取率的各因素分别进行单因素考察,并用响应面法对其进行优化试验。结果:用贝母地上部位25倍量的75%乙醇(v/v)浸泡1.5h后,85℃水浴回流提取2次,每次1.0h为贝母地上部分总生物碱最佳提取条件。结论:本研究提取方法可作为贝母地上部位提取物提取工艺的一种最佳提取方法。
Objective: To study the extraction and purification of extracts of Fritillaria and determine the technical parameters. Methods: The total alkaloids were taken as the investigation index. The total alkaloid content was determined by acid dye colorimetry. The factors affecting the total alkaloid extraction rate were investigated by single factor and optimized by response surface methodology. Results: After soaking in 25% 75% ethanol (v / v) for 1.5h, 85 ℃ water bath was refluxed for 2 times, each time 1.0h was the best extraction condition for the total alkaloids on the aerial part of Fritillaria. Conclusion: The extraction method of this study can be used as the best extraction method for the extraction of the extract of Fritillaria aerial parts.