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用Ｇｌａｓｓｍｉｌｋ法纯化ＰｆＤＮＶｄｓＤＮＡ，在其３′端和ＰｓｔⅠ切开的ｐＵＣ１１９质粒的３′端分别加ｄＧ和ｄＣ尾，连接、转化、筛选得到分子量为８．７ｋｂ的重组质粒。通过酶切证明插入ＤＮＡ为ＰｆＤＮＶ全基因组ＤＮＡ。利用ＤＥＡＥｄｅｘｔｒａｎ转染技术，将此重组质粒导入黑胸大蠊幼虫体内，此重组质粒能使虫体发病死亡。电镜超薄切片观察发现虫体细胞内存在大量的病毒粒子。同样，在免疫扩散实验中虫体ＰＢＳ浸出液能与抗ＰｆＤＮＶ的抗体产生沉淀线。用重组质粒感染致死的虫体喂食健康黑胸大蠊，也能使其发病死亡，通过电镜可以观察到在细胞内增殖的病毒粒子。
The PfDNVdsDNA was purified by the Glassmilk method. The dG and dC tails were respectively added to the 3 ’end of the PstI-cut pUC119 plasmid at the 3’ end and ligated, transformed and screened to obtain a recombinant plasmid with a molecular weight of 8.7 kb. Enzyme digestion proved that the inserted DNA was PfD-NV genome-wide DNA. Using DEAE dextran transfection technology, the recombinant plasmid was introduced into the black-throated Bladderworm larvae, the recombinant plasmid can make the parasite morbidity and mortality. Electron microscopy showed that there were a large number of virus particles in the parasite cells. Similarly, PBS immunoprecipitation in insect lavage fluid can be precipitated with anti-PfDNV antibody lines. Infected with a recombinant plasmid infected lethal worms cockroach can also make its death, by electron microscopy can be observed in the proliferation of intracellular virus particles.