日本最早利用微生物防治害虫是从林业开始的。1933年用白僵菌(B.bassiana)防治松树的毛虫类,用真菌或用虫尸感染。在6个试验点中,幼虫的死亡率在8.6～91.5％之间。 1937、1941年前后,又分离出了金龟子(Anomala testaceipes)幼虫的病原真菌赤僵棒束孢(Isaria kogane)。用含有蚕蛹的培养基培养,进行了较大规模的试验,取得了较好的防治效果。日本农 The earliest use of microorganisms in Japan to control pests is from the forestry. In 1933, pine caterpillar beetles were treated with B. bassiana and infected with fungi or with dead moths. The larval mortality was between 8.6 and 91.5% at six test sites. In 1937, around 1941, the pathogen fungus Isaria kogane was isolated from larvae of Anomala testaceipes. With silkworm pupa culture medium, conducted a large-scale test, and achieved good control effect. Japanese farmers