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目的通过研究细菌毒素和炎症细胞因子诱导的间皮细胞（ＭＣｓ）粘附分子表达及其对腹腔巨噬细胞功能的影响，探讨间皮细胞在腹膜抗菌防御中的作用。方法将体外培养的人腹膜间皮细胞以细菌脂多糖（ＬＰＳ）、ＴＮＦ－α或ＩＬ－１β刺激，采用间接免疫荧光染色技术，于激光扫描共聚焦显微镜下观察间皮细胞表面粘附分子（ＩＣＡＭ－１）的表达；采用细胞瑞氏染色方法，在普通光镜下计数腹腔巨噬细胞的粘附率。结果人腹膜间皮细胞表达ＩＣＡＭ－１，用ＬＰＳ、ＴＮＦ－α或ＩＬ－１β刺激间皮细胞后培养６小时，ＩＣＡＭ－１的表达高于对照组，有显著性差异（Ｐ＜０．０５）；各刺激组腹腔巨噬细胞的粘附率也明显高于对照组（Ｐ＜０．０５）。间皮细胞粘附分子的表达与腹腔巨噬细胞的粘附率呈正相关（ｒ＝０．９１８）。结论炎症介质和促炎症细胞因子通过刺激腹膜间皮细胞粘附分子表达增加，增强腹腔巨噬细胞的粘附和吞噬，在腹膜抗菌防御中起重要作用
Objective To study the effect of mesangial cells on peritoneal antimicrobial defense by studying the expression of adhesion molecules induced by bacterial toxins and inflammatory cytokines and their function on peritoneal macrophages. Methods Human peritoneal mesothelial cells cultured in vitro were stimulated with lipopolysaccharide (LPS), TNF-α or IL-1β by indirect immunofluorescence staining. The adhesion molecules on the surface of mesothelial cells were observed by laser scanning confocal microscopy ICAM-1) expression; using Wright’s staining method, counting the adhesion rate of peritoneal macrophages under the ordinary light microscope. Results ICAM-1 was expressed in human peritoneal mesothelial cells, and the ICAM-1 expression was significantly higher than that in control group after stimulated with LPS, TNF-α or IL-1β for 6 hours (P <0.05 ). The adhesion rate of peritoneal macrophages in each stimulation group was also significantly higher than that in the control group (P <0.05). The expression of mesothelial cell adhesion molecules was positively correlated with the adhesion rate of peritoneal macrophages (r = 0.918). Conclusions Inflammatory mediators and proinflammatory cytokines play an important role in peritoneal antimicrobial defense by stimulating the expression of adhesion molecules in peritoneal mesothelial cells and enhancing the adhesion and phagocytosis of peritoneal macrophages.