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目的:研究异体的EBV-LMP2表位多肽所激活的特异性CTL对具有相同HLA-Ⅰ类分子亚型鼻咽癌细胞(CNE2) 移植瘤的抑制作用,探讨异体间的免疫治疗。方法:先将CNE2细胞接种于Scid鼠背部皮下建立移植瘤模型;然后通过SSP- PCR确定CNE2的HLA-Ⅰ类分子亚型,并选取与CNE2有相同HLA-Ⅰ类分子亚型的外周血淋巴细胞;实验再将3组不同细 胞分别接种于Scid鼠北部皮下,(1)对照组:仅接种CNE2细胞;(2)T细胞组:接种未激活的淋巴细胞与CNE2混合细胞。 (3)CTL组接种经EBV-LNP2多肽激活的特异性CTL与CNE2混合细胞。观察EBV-LMP2多肽激活的CTL对CNE2移植瘤生 长的影响。结果:CNE2于一周左右在Scid鼠背部形成肿块,病理学鉴定为低分化上皮癌;SSP-PCR显示CNE2含有HLA-A11 基因位点;含有相同基因位点的DC负载LMP2-A11多肽所激活的CTL,明显抑制CNE2移植瘤生长。结论:EBV-LMP2多肽 所激活的特异性CTL抑制HLA亚型相同的CNE2移植瘤的形成。
OBJECTIVE: To study the inhibitory effect of specific CTLs activated by allogeneic EBV-LMP2 epitope peptides on the transplanted tumor of CNE2 cells with the same HLA-Ⅰ subtype and to explore the immunotherapy among allogeneic. METHODS: CNE2 cells were inoculated subcutaneously in the back of Scid mice to establish a transplanted tumor model. The subtype of HLA-Ⅰ of CNE2 was identified by SSP-PCR and the peripheral blood lymphoid of the same HLA-Ⅰ subtype as CNE2 Three groups of different cells were inoculated subcutaneously in the north of Scid mice respectively. (1) Control group: CNE2 cells were inoculated only; (2) T cell group: inoculated with mixed lymphocytes and CNE2 cells. (3) The CTL group was inoculated with the mixed CTL and CNE2 cells activated by EBV-LNP2 polypeptide. To observe the effect of CTL activated by EBV-LMP2 polypeptide on the growth of CNE2 xenografts. Results: CNE2 formed a lump in the back of Scid rats in about one week, and pathologically identified as poorly differentiated epithelial carcinoma. SSP-PCR showed that CNE2 contained the HLA-A11 locus; activated by DC-loaded LMP2-A11 polypeptide containing the same locus CTL significantly inhibited the growth of CNE2 xenografts. Conclusion: The specific CTL activated by EBV-LMP2 polypeptide inhibits the formation of CNE2 xenografts with the same HLA subtype.