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胎儿晚期占优势的血红蛋白F于出生一年后就降到很低的水平。成人一般少于2％。以往关于血红蛋白F的研究,大部分是在儿童中进行的。本文报告了成人各种骨髓疾患的观察结果。血红蛋白F测定方法如下:(1)加1/12当量氢氧化钠1.8毫升到0.1毫升溶血中使之碱化,再加4.2毫升27％酸性硫酸铵溶液中和,并校正pH至7.3～7.6;(2)每分钟2500转离心10分钟去除蛋白沉淀物,在pH8.6 TEB缓冲液中,于乙酸纤维素上进行血红蛋白电泳,用Betke-Kleihaur技术进行周围血液涂片的酸洗脱研究。 40例成人对照者血红蛋白F正常值为1.0～3.1％。增生性或恶性骨髓疾病144例中81例、再生不良性贫血7例中5例和1例原发性血小板减少性紫癜者,血红蛋白F均升高。良性骨髓疾病41例患者的血红蛋白F皆正常。
Fetal hemoglobin F, which is predominant in the late fetus, is reduced to a very low level one year after birth. Adults generally less than 2%. Previous research on hemoglobin F has been mostly done in children. This article reports the findings of various bone marrow disorders in adults. The hemoglobin F was determined as follows: (1) Alkalize 1.8 ml of 1/12 equivalent sodium hydroxide to 0.1 ml hemolysis, neutralize with 4.2 ml of 27% acidic ammonium sulfate solution and correct the pH to 7.3-7.6. (2) Centrifugation at 2500 rpm for 10 minutes to remove protein precipitates, hemoglobin electrophoresis on cellulose acetate in pH8.6 TEB buffer, and acid washout of peripheral blood smear using Betke-Kleihaur technique. 40 cases of adult controls hemoglobin F normal 1.0 ~ 3.1%. 81 cases of proliferative or malignant bone marrow disease in 144 cases, 7 cases of aplastic anemia in 5 cases and 1 cases of idiopathic thrombocytopenic purpura hemoglobin F were increased. 41 cases of benign bone marrow disease hemoglobin F are normal.