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国外报道部分慢性肝炎患者存在一种自身抗体—抗微粒体抗体。本文报道乙型肝炎患者抗微粒体抗体检测结果。 对象和方法 一、病例:共检测86例住院肝炎患者血清,其中HBsAg阳性39例;HBsAg阴性/抗-HBc阳性30例;非乙肝感染11例。 二、方法:抗微粒体抗体检测:采用间接免疫荧光方法。抗微粒体抗体阳性对照血清系Dr.Rizze-tto实验室提供;阴性对照包括用正常人血清取代病人血清及用荧光标记羊抗兔IgG取代荧光标记羊抗入IgG。肝内HBsAg、HBcAg检查采用ABC
Foreign reports in some chronic hepatitis patients there is an autoantibody - anti-microsomal antibodies. This article reports hepatitis B patients anti-microsomal antibody test results. Subjects and methods First, the case: a total of 86 cases of hospitalized hepatitis serum were detected, of which 39 cases of HBsAg positive; HBsAg negative / anti-HBc positive in 30 cases; non-hepatitis B infection in 11 cases. Second, the method: anti-microsomal antibody detection: Indirect immunofluorescence method. The anti-microsomal antibody positive control serum was provided by Dr. Rizze-tto Laboratories; negative controls included the replacement of patient serum with normal human serum and the replacement of fluorescently labeled goat anti-IgG with fluorescently labeled goat anti-rabbit IgG. Intrahepatic HBsAg, HBcAg examination using ABC