[目的]建立半夏细胞悬浮培养体系。[方法]以半夏叶柄为外植体,采用正交试验设计研究了2,4-D、NAA、毒莠定和KT4种植物生长物质及其配比对疏松愈伤组织形成的影响。[结果]2,4-D和毒莠定对半夏叶柄愈伤组织诱导效果最显著,KT和NAA次之。诱导半夏叶柄形成疏松愈伤组织的适宜培养基为MS+0.5mg/L2,4-D+1.0mg/LNAA+1.0mg/L毒莠定+1.5mg/LKT。[结论]为通过半夏细胞悬浮提取有效成分和生产人工种子奠定了基础。 [Objective] To establish a cell culture system of Pinellia ternata. [Method] With the petiole of Pinellia ternata as explants, the effects of 2,4-D, NAA, Picloram and KT4 plant growth substances on the formation of loose callus were studied by orthogonal design. [Result] 2,4-D and picloram showed the most significant induction effects on the petiole of the petiole, followed by KT and NAA. Suitable medium for inducing the formation of loose callus in the petiole of Pinellia ternate was MS + 0.5 mg / L, 4-D + 1.0 mg / L NAA + 1.0 mg / L picloram + 1.5 mg / L KT. [Conclusion] It laid the foundation for the extraction of effective components and production of artificial seeds by the suspension culture of Pinellia ternata.