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应用流式细胞仪和免疫组化技术对１２例鼻咽泡状核细胞癌和３３例低分化鳞癌的ＤＮＡ含量及增殖细胞核抗原（ＰＣＮＡ）进行分析。结果显示：泡状核细胞癌的ＤＮＡ指数、非整倍体率和ＰＣＮＡ阳性率均高于低分化鳞癌，表明泡状核细胞癌分化程度低于后者。并初步探讨ＤＮＡ与ＰＣＮＡ所反映的泡状核细胞癌生长生物学特征与预后的联系。Ｐ＜０．０１２．３鼻咽泡状核细胞癌和低分化鳞癌的ＤＮＡ倍体测定见表２。表２泡状核细胞癌和低分化鳞癌ＤＮＡ倍体的测定与低分化鳞癌相比Ｐ＜０．０１３讨论众所周知，ＤＮＡ异倍体是恶性肿瘤的特征，而ＰＣＮＡ可作为细胞增殖状态的一个指标［３］。本文采用流式细胞仪和ＰＣＮＡ免疫组化技术同时对鼻咽泡状核细胞癌的ＤＮＡ含量和ＰＣＮＡ计数进行研究，发现泡状核细胞癌的异倍体发生率为９１．７％，呈现非整倍体或四倍体核型，明显高于低分化鳞癌，后者在ＤＮＡ二倍体／近二倍体核型和非整倍体／四倍体核型的分布大致相等，其ＤＩ值和ＰＣＮＡ阳性率远低于泡状核细胞癌。宗永生等［４］曾报道泡状核癌细胞群体１２例中７例癌细胞众数的ＤＮＡ大于二倍体含量，８例泡状核细胞癌癌细胞总数中其众数癌细胞位于二倍体和四倍体之间，也说明其ＤＮＡ含量大于二倍体，本?
The DNA content and proliferating cell nuclear antigen (PCNA) in 12 cases of nasopharyngeal vesicular cancer and 33 cases of poorly differentiated squamous cell carcinoma were analyzed by flow cytometry and immunohistochemistry. The results showed that the DNA index, aneuploidy rate and PCNA positive rate of GCS were higher than that of poorly differentiated squamous cell carcinoma, indicating that the degree of differentiation of GCs was lower than that of the poorly differentiated squamous cell carcinoma. The relationship between the biological characteristics and the prognosis of the somatic cell carcinoma reflected by DNA and PCNA was also discussed. P <0.012.3 DNA ploidy measurements of nasopharyngeal vesicular and poorly differentiated squamous cell carcinoma are shown in Table 2. Table 2 Determination of DNA Ploidy in Bubble and Poorly Differentiated Squamous Cell P <0.013 Compared with Poorly Differentiated Squamous Cell Carcinoma It is well known that DNA aneuploidy is a hallmark of malignant tumors and that PCNA can be used as a marker for cell proliferation An indicator [3]. In this paper, flow cytometry and PCNA immunohistochemistry at the same time the nasopharyngeal cell carcinoma of the DNA content and PCNA count study found that somatic cell aneuploidy incidence was 91.7%, showing non- Euploids or tetraploids karyotype, significantly higher than poorly differentiated squamous cell carcinoma, the latter in the DNA diploid / nearly diploid karyotype and aneuploid / tetraploid karyotype distribution roughly equal, the DI The positive rates of PCNA and PCNA were much lower than those of goblet cell carcinoma. Zong Yongsheng et al. [4] reported that the DNA of seven cancer cells in the 12 cases of vesicular cancer cells was larger than the diploid, and the number of cancer cells in all the 8 cases of cancerous cells was double Between the body and tetraploid, also shows that its DNA content is greater than diploid, this?