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A new fluorescent probe RY was synthesized for the detection of Au3+ions based on a rhodamine B derivative.The fluorescent probe showed good selectivity and sensitivity to Au3+ions.Obvious color and fluorescence changes could be observed with the naked eye while the fluorescent probe reacted with the Au3+ions.The detection limit of the probe was determined to be 36 ppb by the fluorescence titration.The excellent linear relationship suggests that the probe is potentially useful for quantitative detection of Au3+in vitro.We also demonstrated its bioimaging application in both living cells and mice.This was the first time that a fluorescent probe was successfully applied to imaging Au3+in living animals.
A new fluorescent probe RY was synthesized for the detection of Au3 + ions based on a rhodamine B derivative. The fluorescent probe showed good selectivity and sensitivity to Au3 + ions. Obvious color and fluorescence changes could be observed with the naked eye while the fluorescent probe reacted with the Au3 + ions. The detection limit of the probe was determined to be 36 ppb by the fluorescence titration.The excellent linear relationship suggests that the probe is potentially useful for quantitative detection of Au3 + in vitro. We also demonstrate its bioimaging application in both living cells and mice. This was the first time that a fluorescent probe was successfully applied to imaging Au3 + in living animals.