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为探索与烟叶中茄尼醇含量相关基因的遗传模式,以低茄尼醇含量烟草种质Maryland609为母本、高茄尼醇含量烟草种质K326为父本配制杂交组合,建立P_1、P_2、F_1、F_2的4个世代遗传分析群体,利用UPLC(超高效液相色谱)进行茄尼醇的定量检测,通过“主基因+多基因”混合遗传模型多世代联合分析对与茄尼醇含量相关的基因进行遗传分析。结果表明,Maryland609×K326组合的茄尼醇含量受2对等显性主基因+加性显性多基因(E6)控制,2对主基因的加性效应和显性效应值相等,均为负值,多基因的显性效应大于加性效应;主基因的遗传率分别为33.61%和53.15%,多基因遗传率分别为30.01%和13.64%,环境变异在33.21%~36.28%。因此,主基因和多基因共同决定了烟叶茄尼醇的含量,以主基因遗传为主,同时受到环境的影响。
In order to explore the genetic model of solanesol content related genes in tobacco leaves, Maryland609 with low solanesol content as the female parent and K326 with high solanesol content as the male parent was used as the male parent to establish P_1, P_2, F_1 and F_2, the quantitative analysis of solanesol was carried out by UPLC (Ultra Performance Liquid Chromatography), and the results of multiple generation combined analysis of multiple genes with “main gene + polygene ” showed that solanesol Content-related genes for genetic analysis. The results showed that the content of solanesol in Maryland609 × K326 combination was controlled by two dominant dominant genes + additive dominant polygene (E6), and the additive and dominance effects of the two major genes were equal and both were negative The dominant gene effect of polygene was greater than that of additive effect. The heritability of major genes were 33.61% and 53.15%, respectively. The polygene heritabilities were 30.01% and 13.64%, respectively. The environmental variation ranged from 33.21% to 36.28%. Therefore, the major genes and polygenes jointly determine the content of solanesol in tobacco leaves, which is dominated by the major genes and influenced by the environment at the same time.