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目的:通过观察连翘酯苷A(forsythiaside A,FA)对酵母致热小鼠体温的影响以及体温调节中枢及外周温度敏感瞬时感受器电位离子通道(transient receptor potential,TRP)A1表达影响,明确FA的解热作用及可能的机制。方法:60只雄性C57BL/6小鼠随机平均分为以下6组,分别为正常组,模型组,FA不同剂量组(2,4,8 mg·kg-1)和阳性药阿司匹林组(90mg·kg-1)。除正常组外,其他5组小鼠以背部皮下注射酵母混悬液法建立发热模型,并于造模前以及造模后17~22 h检测小鼠体温情况;造模后22 h处死小鼠,取下丘脑和背根神经节(dorsal root ganglion,DRG),通过免疫组化和Western blot方法检测TRPA1的表达水平。结果:与正常组比较,注射酵母后模型组小鼠体温明显上升,17 h达到高峰期(P<0.01),下丘脑和背根神经节中TRPA1水平显著降低(P<0.01);与模型组比较,FA剂量4,8 mg·kg-1可显著降低酵母引起的小鼠体温升高,上调下丘脑体温调节主要神经核团下丘脑室旁核(PVN)和视上核(SO),以及外周DRG中TRPA1水平(P<0.05,P<0.01)。结论:FA能降低酵母致热小鼠的体温,其作用可能与其对下丘脑和DRG中TRPA1的有效调节有关。
OBJECTIVE: To observe the effect of forsythiaside A (FA) on the body temperature of mice induced by yeast and the expression of temperature-dependent transient receptor potential (TRP) A1 in the central and peripheral temperature, Antipyretic effect and possible mechanism. Methods: Sixty male C57BL / 6 mice were randomly divided into the following 6 groups: normal group, model group, different dose FA groups (2,4,8 mg · kg -1) and positive drug aspirin group (90 mg · kg -1) kg-1). In addition to the normal group, the other 5 groups of mice were subcutaneously injected with yeast suspension to establish a fever model, and the body temperature of the mice was measured before modeling and 17-22 h after modeling; mice were sacrificed 22 h after model establishment The thalamus and dorsal root ganglion (DRG) were removed and the expression of TRPAl was detected by immunohistochemistry and Western blot. Results: Compared with the normal group, the body temperature of the model group increased significantly after 17 h injection of yeast (P <0.01), while the level of TRP A1 in the hypothalamus and dorsal root ganglion decreased significantly (P <0.01) FA doses of 4 and 8 mg · kg-1, respectively, significantly reduced yeast temperature-induced hypothermia in mice, up-regulated hypothalamic-thermoregulatory hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SO) TRPA1 levels in peripheral DRGs (P <0.05, P <0.01). Conclusion: FA can reduce the temperature of yeast-induced fever in mice, which may be related to its regulation of TRPAl in the hypothalamus and DRG.