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目的探讨无名异冲剂治疗骨折的作用机理。方法新西兰家兔５５只造成双侧桡骨３ｍｍ缺损的骨折后随机分为用药组和对照组，于术后１、２、３、４、５周取骨痂制成石蜡切片，以Ｆｅｕｌｇｅｎ反应和免疫组化技术分别显示ＤＮＡ和ＢＭＰ，进行图像定量分析。结果在骨折愈合过程中，骨痂中骨折修复细胞ＤＮＡ指数和骨痂局部ＢＭＰ含量的总体变化趋势两组相同；用药组第１、２周骨痂ＤＮＡ指数和ＢＭＰ含量明显高于同期的对照组（Ｐ＜００１）；用药组ＢＭＰ高峰出现在第２周，对照组出现在第３周。结论无名剂冲剂能促进骨折修复细胞的增殖，增加成骨细胞的活性，诱导ＢＭＰ合成，加速骨折愈合。
Objective To explore the mechanism of the treatment of fractures with Wuming Granules. METHODS: Fifty-five New Zealand rabbits were randomly divided into two groups: the drug group and the control group. Fistulas were made at week 1, 2, 3, 4 and 5 postoperatively to obtain paraffin sections. Feulgen reaction and immunization were performed. The quantification technology showed DNA and BMP, respectively, for quantitative image analysis. Results During the fracture healing process, the overall change trends of DNA repair index and BMP content in bone callus were similar in both groups; the DNA index and BMP content of callus were significantly higher in the first and second weeks of treatment group than in the control group at the same time. (P<001); The peak of BMP appeared in the second week in the medication group and the third week in the control group. Conclusion Anming agent granules can promote the proliferation of fracture repair cells, increase the activity of osteoblasts, induce BMP synthesis, and accelerate fracture healing.