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目的观察二氮嗪预处理对在体大鼠心肌缺血再灌注损伤(IRI)的保护作用。方法健康雄性SD大鼠36只,随机分成4组,每组9只。采用结扎左冠状动脉前降支(LAD)法制备心肌IRI模型。记录缺血再灌注前后不同时间点心功能指标,TTC染色测定心肌梗塞面积,检测血清CK-MB及LDH活性。结果结扎左冠状动脉前降支后:IRI组的CK-MB和LDH显著高于Sham组(P<0.05);IPC和DPC组较IRI组显著下降(P<0.05)。与IRI组比较,IPC组及DPC组梗死面积明显降低(P<0.05)。缺血30min、再灌注60min、120min时,IRI组、IPC组和DPC组与Sham组比较,±dp/dtmax、LVSP、LVEDP有显著的差异(P<0.05);IPC组和DPC组与IRI组比较,±dp/dtmax、LVSP差别有统计学意义(P<0.05)。结论二氮嗪预处理对在体大鼠缺血再灌注损伤心肌具有保护作用,可降低血清心肌酶含量、减少心肌梗死面积,维护心肌收缩和舒张功能。
Objective To observe the protective effect of diazoxide preconditioning on myocardial ischemia-reperfusion injury (IRI) in vivo. Methods Thirty-six healthy male Sprague-Dawley rats were randomly divided into 4 groups with 9 rats in each group. Myocardial IRI model was prepared by ligating left anterior descending coronary artery (LAD). The indexes of cardiac function at different time points before and after ischemia-reperfusion were recorded. The area of myocardial infarction was measured by TTC staining, and the activities of serum CK-MB and LDH were measured. Results After ligation of the left anterior descending coronary artery, the CK-MB and LDH in IRI group were significantly higher than those in Sham group (P <0.05). The IPC and DPC group were significantly lower than IRI group (P <0.05). Compared with IRI group, infarct size in IPC group and DPC group was significantly lower (P <0.05). There were significant differences in ± dp / dtmax, LVSP and LVEDP between IRI group, IPC group and DPC group at 60min and 120min after reperfusion (P <0.05) The difference between ± dp / dtmax and LVSP was statistically significant (P <0.05). Conclusion Diazoxide preconditioning can protect myocardium from ischemia-reperfusion injury in vivo, decrease the content of serum myocardial enzymes, decrease the area of myocardial infarction, and maintain myocardial contractility and diastolic function.