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养猪沼液氮磷等营养物质丰富,可作为廉价的螺旋藻培养基,但其成分复杂,尤其是高氨氮等因素严重抑制螺旋藻的生长.采用序批式生物反应器(sequencing batch reactor,SBR)降低沼液中的氨氮浓度,通过改变进水中化学需氧量(chemical oxygen demand,COD)与总氮(total nitrogen,TN)的比值,研究了沼液中的亚硝态氮及硝态氮的保留情况,为螺旋藻生长提供氮源.通过对比螺旋藻在不同工况出水中的生长情况,以及氮元素的保留情况,筛选出最佳SBR工况.摇瓶试验结果表明,当进水COD/TN=3.0,出水中氨氮、硝态氮、亚硝态氮浓度分别为51.2、91.6、213.1 mg·L~(-1),此时螺旋藻具有较快生长速率,产率达到0.084 g·(L·d)~(-1).在此基础之上,通过放大螺旋藻培养规模至120L,研究了螺旋藻在室外大棚中的生长情况及螺旋藻对沼液中氮、磷元素的去除,结果表明螺旋藻在室外依然生长良好,培养10 d后,产率为(0.075±0.003)g·(L·d)~(-1),螺旋藻蛋白含量达到60%左右,养殖出水中氨氮去除率达到99%.
Nitrogen and phosphorus in pig’s biogas slurry is rich in nutrients, which can be used as a cheap medium for Spirulina, but its composition is complex, especially high ammonia nitrogen and other factors seriously inhibit the growth of Spirulina.Sequencing batch reactor SBR) to reduce the concentration of ammonia nitrogen in biogas slurry. By changing the ratio of chemical oxygen demand (COD) to total nitrogen (TN) in the biogas slurry, the nitrite nitrogen and nitrate State of nitrogen retention, to provide a source of nitrogen for the growth of spirulina .Spirulina species in different conditions by the growth of water conditions, as well as the retention of nitrogen, screened the best SBR conditions.The shake flask test results show that when The influent COD / TN = 3.0, the concentrations of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the effluent were 51.2, 91.6 and 213.1 mg · L -1, respectively. Spirulina had faster growth rate 0.084 g · (L · d) -1. On the basis of this, the growth of Spirulina platensis in greenhouse was studied by means of enlarging the culture scale of Spirulina platensis to 120L and the effect of Spirulina platensis on nitrogen and phosphorus The results showed that spirulina still grew well in the outdoor, and the yield was (0.075 ± 0.003) g · L · d -1, the protein content of spirulina reached about 60%, and the ammonia nitrogen removal rate reached 99% in the culture effluent.