论文部分内容阅读
用传统的吸收光谱法测量全血血红蛋白,即使稀释于合适的缓冲剂中,也难以得到满意的结果,原因为全血对光有强敖射。光声光谱法对散射光不敏感,样品无需特殊的制备,即可对固体、液体和凝胶体等进行测量。全血涂片的紫外、可见谱段光声光谱和血红蛋白的吸收光谱同样清楚地显示了含氧血红蛋白(Hb)的特征谱:420nm处的γ吸收峰、550nm处的β峰和585am处的α峰。本文首次报道了将光声光谱应用于仔猪缺铁性贫血研究的一个阶段性结果。
Measurement of whole blood hemoglobin by conventional absorption spectroscopy is difficult to obtain with satisfactory results even when diluted in a suitable buffer due to the strong AO2 emission of whole blood on light. Photoacoustic spectroscopy is insensitive to stray light, and samples can be measured on solids, liquids and gels without special preparation. Ultraviolet, visible spectral photoacoustic spectroscopy and hemoglobin absorption spectra of whole blood smears also clearly show the characteristic spectra of oxygenated hemoglobin (Hb): the γ absorption at 420 nm, the β at 550 nm and the α at 585 am. This paper reports for the first time a phase-by-phase result of applying photoacoustic spectroscopy to iron deficiency anemia in piglets.