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目的:探讨纳米银(nanosilver,Nano-Ag)对1型单纯疱疹病毒(herpes simplex virus-1,HSV-1)的抑制作用。方法:通过MTT分析法、倒置显微镜下观察细胞病变效应(cytopathic effect,CPE)、透射电镜观察细胞形态以及流式细胞仪检测细胞的凋亡,探讨Nano-Ag对HSV-1的抑制作用。结果:Nano-Ag溶液在人宫颈癌细胞(human cervical cancer cells,HeLa)中的最大无毒浓度(TD0)为50μg.mL-1;HSV-1在HeLa细胞的半数感染浓度(50%tissue culture infectious dose,TCID50)为10-4.5.100μL-1;100μg.mL-1Nano-Ag溶液和等体积的400 TCID50HSV-1毒株在室温下充分混和作用2 h后感染HeLa细胞,CPE为阴性,细胞存活率为(101.48±2.29)%,细胞凋亡率为(8.47±1.96)%,电镜下细胞形态良好;而200 TCID50HSV-1毒株感染HeLa细胞,细胞存活率仅为(17.13±2.20)%,CPE为++++,细胞凋亡率为(25.72±1.37)%,二者比较具有显著的统计学差异(P<0.001)。结论:Nano-Ag对HSV-1具有明显的抑制作用。
Objective: To investigate the inhibitory effect of nanosilver (Nano-Ag) on herpes simplex virus-1 (HSV-1). Methods: MTT assay was used to observe the cytopathic effect (CPE) under inverted microscope. The cell morphology was observed by transmission electron microscopy and the apoptosis was detected by flow cytometry. The inhibitory effect of Nano-Ag on HSV-1 was also investigated. Results: The maximum non-toxic concentration (TD0) of Nano-Ag solution in human cervical cancer cells (HeLa) was 50μg.mL-1. The half-infection concentration of HSV-1 in HeLa cells infectious dose (TCID50) of 10-4.5.100μL-1. After infection with 100μg.mL-1Nano-Ag solution and an equal volume of 400 TCID50HSV-1 strain at room temperature for 2 hours, HeLa cells were infected with CPE, The survival rate was (101.48 ± 2.29)% and the apoptosis rate was (8.47 ± 1.96)%. The cell morphology was good under electron microscope. However, the infection rate of HeLa cells was only (17.13 ± 2.20)% , CPE was ++++, apoptosis rate was (25.72 ± 1.37)%, there was a significant statistical difference between the two (P <0.001). Conclusion: Nano-Ag has a significant inhibitory effect on HSV-1.