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本文通过生物降解和摩擦学试验研究了脂肪酰基胱氨酸对石蜡矿物润滑油生物降解和摩擦学性能的影响,通过观察石蜡油降解过程中微生物菌体形态变化分析了添加剂促进润滑油生物降解的机理.结果表明:脂肪酰基胱氨酸能显著地提高石蜡基础油的生物降解性能,有效提高基础油的承载能力和减摩抗磨性能.试验菌种B1502在不含添加剂的石蜡油培养液中12 h即有芽孢形成,48 h菌体全部转变为芽孢;在含添加剂的石蜡油培养液中,试验菌种168 h始终保持营养体的形态,未观察到芽孢形成.添加剂促进石蜡油生物降解的作用机理是为降解润滑油的微生物提供前期生长所需的营养成分以维持其旺盛的生长状态,使中后期石蜡油分解代谢相关酶的表达水平或活性增加,起到促进石蜡油生物降解的作用.
In this paper, the biodegradation and tribology experiments were conducted to study the effect of fatty acyl cystine on the biodegradation and tribological properties of paraffin mineral oil. Through observing the morphological changes of microbial cells during the degradation of paraffin oil, the effects of additives on the biodegradation of lubricants Mechanism.The results showed that fatty acyl cystine can significantly improve the biodegradability of paraffinic base oil and effectively improve the carrying capacity and anti-friction and anti-wear properties of the base oil.Experimental strain B1502 in paraffin oil medium without additives Spores formed after 12 h, and all spores were transformed into spores after 48 h. In the paraffin oil medium with additives, the test strains maintained the shape of vegetative bodies for 168 h and no spore formation was observed. The additives promoted the biodegradation of paraffin oil The mechanism of action is to provide lubricating oil microorganisms to provide pre-growth nutrients needed to maintain its vigorous growth state, so that mid-late paraffin oil catabolism related enzymes expression level or activity increased, to promote the biodegradation of paraffin oil effect.