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目的探讨金花菜对营养性肥胖大鼠体重及血脂的影响及可能作用机制。方法采用高脂饲料喂养9周建立营养性肥胖大鼠模型,将50只造模成功大鼠随机分为模型对照组、阳性对照组和金花菜高、中、低剂量组,每组10只,另设正常大鼠10只为空白对照组。金花菜高、中、低剂量组分别给予金花菜混悬液2、1、0.5 g/(kg·d)灌胃,阳性对照组给予奥利司他胶囊35 mg/(kg·d)灌胃,空白对照组、模型对照组给予同体积(15 ml/kg)的蒸馏水灌胃,连续6周。于给药前后测量大鼠体重计算总增重,给药后检测血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)水平和肝脏卵磷脂胆固醇脂酰转移酶(LCAT)、胆固醇7α-羟化酶(CYP7A1)、过氧化物增殖激活物受体α(PPARα)mRNA和蛋白表达。结果与模型对照组比较,各给药组大鼠给药后体重及总增重明显下降,金花菜高剂量组和阳性对照组TC、TG和LDL-C明显降低,HDL-C显著升高(P<0.05或P<0.01)。治疗后各组肝脏组织PPARα和CYP7A1 mRNA表达差异无统计学意义(P>0.05)。与模型对照组比较,金花菜高剂量组PPARα蛋白表达升高,而阳性对照组PPARα、LCAT的蛋白表达显著降低(P<0.05或P<0.01)。结论金花菜对营养性肥胖大鼠有较好的减肥降脂作用,其机制可能与激活PPARα蛋白表达有关。
Objective To investigate the effects of flowering ginseng on the body weight and blood lipids in obese rats and its possible mechanism. Methods The model of nutritional obesity rats was established by feeding with high-fat diet for 9 weeks. Fifty successful rats were randomly divided into model control group, positive control group and high, medium and low dose group of 10: Another 10 normal rats as blank control group. The high, medium and low dose groups were given gavage suspension 2,1,0.5 g / (kg · d) gavage, the positive control group was given Orlistat 35 mg / (kg · d) , Blank control group and model control group were given the same volume (15 ml / kg) of distilled water for 6 weeks. The weight of rats was measured before and after administration to calculate the total body weight. Serum triglyceride (TG), low density lipoprotein cholesterol (LDL-C), total cholesterol (TC), high density lipoprotein cholesterol (HDL- C) levels and liver lecithin cholesterol acyltransferase (LCAT), cholesterol 7α-hydroxylase (CYP7A1), peroxisome proliferator-activated receptor α (PPARα) mRNA and protein expression. Results Compared with the model control group, the body weight and total body weight of the rats in each administration group decreased significantly. The levels of TC, TG and LDL-C in Hippogamna high dose group and positive control group were significantly decreased, and HDL-C was significantly increased P <0.05 or P <0.01). There was no significant difference in the expression of PPARα and CYP7A1 mRNA in each group after treatment (P> 0.05). Compared with the model control group, the expression of PPARαprotein in high-dose group increased, while the protein expression of PPARαand LCAT in the positive control group was significantly decreased (P <0.05 or P <0.01). Conclusions: Cauliflower has good effect on reducing weight and lipid in nutrition obese rats, which may be related to the activation of PPARα protein expression.