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主要研究矿质元素对北虫草继代培养中形成的不同类型菌落子实体产量及菌丝生长速度的影响,旨在探讨矿质元素与子实体产量以及菌丝生长速度的关系。采用正交试验,将北虫草优良菌株L20在不同矿质元素培养基上进行12次继代培养。结果表明菌株L20在不同培养基中继代培养后形成7种菌落类型,有子实体形成能力的菌落为Ⅰ、Ⅱ、Ⅲ、Ⅴ型,其中Ⅰ型菌落产量最高、Ⅲ型中等、Ⅱ型最低,Ⅴ型形成的子实体均为畸形;Ⅳ、Ⅵ、Ⅶ型无子实体形成能力。菌丝生长速度依次为Ⅴ型>Ⅳ型>Ⅶ型>Ⅵ型>Ⅲ型>Ⅱ型>Ⅰ型,高产型菌落生长速度最慢,而低产型菌落菌丝徒长,表明菌落生长速度与子实体产量成负相关。优型菌落——Ⅰ型菌落子实体高产及高产保持代数最多的矿质元素配方为K3Mg2Ca4Mn3Zn2,其中Mn和K促进子实体高产,K、Mg、Ca有利于高产保持多代。抑制Ⅰ型菌落菌丝徒长的最佳配方为K3Mg2Ca2Mn3Zn2,其中Mn对其抑制作用最为显著。
This paper mainly studies the effects of mineral elements on the yield and mycelial growth rate of different types of colony formed in the subculture of Cordyceps militaris. The aim is to discuss the relationship between mineral elements and fruiting body yield and mycelial growth rate. Using orthogonal test, the excellent strain of Cordyceps militaris L20 was subcultured 12 times on different mineral elements medium. The results showed that strain L20 was subcultured in different media to form seven types of colonies. The colonies with fruiting body formation ability were type Ⅰ, Ⅱ, Ⅲ and Ⅴ. Among them, type Ⅰ colonies had the highest yield, medium type Ⅲ and lowest type Ⅱ , Ⅴ type of fruiting bodies are deformed; Ⅳ, Ⅵ, Ⅶ type of sub-entity-forming ability. The growth rate of mycelium was V type> Ⅳ type> Ⅶ type> Ⅵ type> Ⅲ type> Ⅱ type> Ⅰ type. The growth rate of high yielding colony was the slowest, while the low yielding colony mycelium growth was slow, indicating that the colony growth speed and fruiting bodies Production is negatively correlated. The best type colony-type I colony fruiting body high-yield and high-yield maintaining algebra is the most mineral element formula K3Mg2Ca4Mn3Zn2, in which Mn and K promoter fruiting bodies high yield, K, Mg, Ca is conducive to high-yield to maintain multiple generations. The best formula for inhibiting the growth of type I colonies was K3Mg2Ca2Mn3Zn2, of which Mn had the most significant inhibitory effect.