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以大曹杏、意大利7号、泾阳梅杏三个抗寒性不同的杏品种为试材,经不同低温(盛开花为:0℃、-1℃、-3℃、-5℃、-6℃、-8℃;花蕾为- 2℃、-3℃、-5℃、-7℃、-9℃、-11℃)胁迫5 h后测定杏花的 SOD活性和细胞质膜透性及丙二醛(MDA)含量,观察褐变情况。结果表明:随着处理温度的降低,细胞质膜透性明显加剧,MDA含量呈规律性的变化,SOD活性先上升后下降,且营期和盛花期表现一致,说明SOD活性、MDA含量和细胞质膜透性均可做为抗寒性鉴定的指标。
The apricot cultivars with different cold resistance, such as Da Cao Cao, Italy 7 and Jing Yang Mei Xing, were used as test materials. After different low temperature (flowering: 0 ℃, -1 ℃, -3 ℃, -5 ℃, -6 ℃, -8 ℃; buds of -2 ℃, -3 ℃, -5 ℃, -7 ℃, -9 ℃, -11 ℃) for 5 h after the determination of SOD activity and cell membrane permeability of almond and two Aldehyde (MDA) content, browning observed. The results showed that with the decrease of treatment temperature, the permeability of plasma membrane increased significantly, the content of MDA changed regularly, the activity of SOD increased at first and then decreased, and the performance at camp and full flowering stage showed that SOD activity, MDA content and plasma membrane Permeability can be used as an indicator of cold resistance.