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目的 通过对人工养殖蛙及其池水中O1群霍乱弧菌检测,了解霍乱弧菌在其中的存活及菌型分布情况,为制定防治措施提供依据。 方法 采集人工养殖蛙及养蛙池水进行霍乱弧菌分离培养,血清学诊断符合O1群霍乱弧菌者,按国标法进行系统菌株鉴定。 结果 从624份标本中检出51株O1群E1-Tor霍乱弧菌,总检出率为8.17%,其中稻叶型42株,占 82.4%,小川型 9 株,占 17.6%;噬菌体—生物分型:5K29 株,占 56.9%,其次为 9L8 株、5L5 株、13K5株、9K2株、12K2株。 结论 检出51株O1群 E1-Tor霍乱弧菌均属非流行株。池水检出率高于蛙的检出率。总检出率较高,提示在今后霍乱监测中,这些环境应作为重点之一。
Objective To detect Vibrio cholerae Vibrio cholerae (O1) Vibrio cholerae in artificial breeding frogs and their water samples, and to find out the survival and distribution of Vibrio cholerae in them, so as to provide basis for the prevention and control measures. Methods Vibrio cholerae were isolated and cultured by artificially cultured frogs and frog ponds. Serogroups were identified as Vibrio cholerae serogroup O1 and identified according to the national standard method. Results A total of 51 Vibrio cholerae isolates were identified from 624 samples. The total detection rate was 8.17%, of which 42 strains were rice leaf type (82.4%) and 9 strains were Ogawa type (17.6%). Phage - Typing: 5K29 strains, accounting for 56.9%, followed by 9L8 strains, 5L5 strains, 13K5 strains, 9K2 strains, 12K2 strains. Conclusion 51 isolates of E1-Tor Vibrio cholerae were detected as non-epidemic strains. The detection rate of water pool is higher than the detection rate of frogs. The overall detection rate is high, suggesting that these environments should be one of the priorities in future cholera surveillance.