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目的：探讨人乳头状瘤病毒（ＨＰＶ）感染在食管鳞状细胞癌（ＥＳＣＣ）发生发展中的意义。方法：采用原位杂交和免疫组化ＬＳＡＢ法检测３３例ＥＳＣＣ中的ＨＰＶＤＮＡ、ＨＰＶ属特异性结构抗原（ＨＰＶＧＳＡｓ）和增殖细胞核抗原（ＰＣＮＡ）。结果：３３例ＥＳ－ＣＣ中的８例可见ＨＰＶＤＮＡ阳性细胞，阳性率达２４．２％；这些ＨＰＶＤＮＡ阳性细胞的ＰＣＮＡ均阴性，３３例ＥＳＣＣ，包括８例有ＨＰＶＤＮＡ阳性细胞者，ＨＰＶＧＳＡｓ均为阴性。在癌旁增生、异型增生和原位癌灶中未见ＨＰＶＤＮＡ或ＨＰＶＧＳＡｓ阳性细胞。结论：ＨＰＶ感染可能在某些ＥＳＣＣ的进展过程中起到一定的作用
Objective: To investigate the significance of human papillomavirus (HPV) infection in the development of esophageal squamous cell carcinoma (ESCC). Methods: HPVDNA, HPV genus-specific structural antigen (HPVGSAs) and proliferating cell nuclear antigen (PCNA) were detected in 33 cases of ESCC by in situ hybridization and immunohistochemistry. Results: The positive rate of HPVDNA positive cells was 24.2% in 8 out of 33 cases of ES-CC. The PCNA of these HPVDNA positive cells were all negative and 33 cases of ESCC, including 8 cases of HPVDNA positive cells, HPVGSAs were negative . In the paracancerous hyperplasia, dysplasia and in situ foci no HPVDNA or HPVGSAs positive cells. Conclusion: HPV infection may play a role in the progress of some ESCCs