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目的初步探讨放射复合伤对骨髓造血微环境中基质细胞粘附功能损伤的机理。方法采用流式细胞仪、细胞粘附实验和细胞粘附阻断实验等方法，检测５０Ｇｙγ射线全身照射、１５％体表面积Ⅲ度烧伤和放射复合伤小鼠骨髓基质细胞表达血管细胞粘附分子（ＶＣＡＭ１）、纤维连接素（Ｆｎ）、层粘素（Ｌｎ）和Ⅳ型胶原（ＣｏｌⅣ）等成分的变化以及骨髓基质细胞贴壁层对骨髓单个核细胞粘附能力的影响。结果①骨髓基质细胞表达ＶＣＡＭ１、Ｆｎ、Ｌｎ和ＣｏｌⅣ的水平具有以单烧组＞正常组＞复合伤组＞单放组的特点。②伤后第３～７天，单烧组骨髓基质细胞的粘附能力显著高于正常组（Ｐ＜００５～Ｐ＜００１）；单放组和伤后第３天的复合伤组则反之。③分别用ＶＣＡＭ１、Ｆｎ、Ｌｎ和ＣｏｌⅣ单抗处理骨髓基质细胞贴壁层后，骨髓单个核细胞的粘附率较未加单抗组均有不同程度的下降。结论骨髓微环境基质细胞粘附功能的损伤可能是影响放射复合伤骨髓造血功能障碍的原因之一。
OBJECTIVE: To investigate the mechanism of radiation injury on stromal cell adhesion in bone marrow hematopoietic microenvironment. Methods Flow cytometry, cell adhesion assay and cell adhesion inhibition assay were used to detect the expression of vascular cell adhesion in 5 GyGamma irradiation, 15% body surface area of third degree burn and radiation combined bone marrow stromal cells (VCAM-1), fibronectin (Fn), laminin (Ln) and collagen Ⅳ (Col Ⅳ) and other components of the changes of bone marrow stromal cell adhesion layer on bone marrow mononuclear cell adhesion capacity. Results ① The expression of VCAM1, Fn, Ln and ColⅣ in bone marrow stromal cells had the characteristics of single burn group> normal group> complex injury group. ② On the 3rd to 7th days after injury, the adhesive ability of BMSCs in single burn group was significantly higher than that in normal group (P <005 ~ P <001); in single burn group and on the 3rd day after injury The group on the contrary. (3) The adhesive rate of bone marrow mononuclear cells decreased with different degrees after treatment of adherent layer of bone marrow stromal cells with VCAM-1, Fn, Ln and Col IV monoclonal antibody respectively. Conclusion Bone marrow microenvironment stromal cell adhesion injury may be one of the reasons that affect the bone marrow dysfunction of radiation combined injury.