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目的:探讨酶联免疫法(ELISA)检测唾液中幽门螺杆菌抗体在诊断幽门螺杆菌感染中的临床效果。方法:ELISA检测98例幽门螺杆菌感染患者唾液中的幽门螺杆菌抗体,并与其它三种常规方法比较,评价ELISA检测效果。结果:ELISA法检测幽门螺杆菌阳性73例,幽门螺杆菌阴性25例,RUT检测阳性72例,阴性26例,HE染色检测阳性72例,阴性26例,WS银染色法检测阳性73例,阴性25例,ELISA法与其他三种常规方法结果比较差异均无统计学意义(P>0.05)。结论:ELISA检测唾液中的幽门螺杆菌抗体具有较高的灵敏度和特异性,且操作方法简单。
Objective: To investigate the clinical efficacy of ELISA for the detection of Helicobacter pylori in saliva for the diagnosis of Helicobacter pylori infection. Methods: ELISA was used to detect Helicobacter pylori antibody in saliva of 98 patients with H. pylori infection and compared with the other three routine methods to evaluate the effect of ELISA. Results: There were 73 positive cases of Helicobacter pylori, 25 negative cases of Helicobacter pylori in ELISA test, 72 positive cases and 26 negative cases by HE staining, 72 cases were positive by HE staining, 26 cases were negative, and 73 cases were negative by WS silver staining There was no significant difference between the 25 cases and the other three methods (P> 0.05). Conclusion: ELISA detection of saliva in Helicobacter pylori antibody with high sensitivity and specificity, and the operation method is simple.