大多数养蜂者对美洲幼虫腐臭病极为担忧,因为有很多州规定病群要烧毁。这种病能够通过饲喂受幼虫芽孢杆菌(Bacillus larvea)孢子污染的蜂蜜传播。我们利用这个改进的方法检验蜂蜜样品,从不同来源的58个样品中,发现33个有幼虫芽孢杆菌。幼虫芽孢杆菌对人是否有害,现在还不清楚。以往蜂蜜带孢子的最低测定水平大概是每毫升6000个孢子。采取我们的方法,现在有可能(?)每毫升蜂蜜中含80个孢子进行测定。 Most beekeepers are extremely worried about American larvae as they are burned down by many states. The disease can be spread by feeding honey contaminated with Bacillus larvea spores. We tested honey samples using this improved method, and 33 of the 58 samples from different sources found Bacillus larvae. Whether larvae Bacillus is harmful to humans is still unclear. In the past, honey had the lowest spore determination level of about 6,000 spores per ml. Taking our approach, it is now possible to determine (?) 80 spores per ml of honey.