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为探讨TiERF1和RC7基因对小麦抗全蚀病的防御反应,本研究对转TiERF1-RC7双价基因小麦进行了分子检测以及全蚀病抗性的室内和田间鉴定。结果表明,转入的TiERF1和RC7基因在转基因小麦中可以遗传和转录;与受体扬麦18相比,5个转TiERF1-RC7小麦株系在整个生育期抗病性显著提高,苗期的全蚀病严重度在10%以下,成熟期的白穗率在13%以下,而扬麦18的严重度为62.98%,白穗率为26.09%。电子显微镜观察结果表明抗病转基因小麦根表的全蚀病原菌菌丝数量及生长势明显低于感病材料。上述结果说明,转入的TiERF1和RC7基因抑制了全蚀病原菌的侵染及在转基因小麦中繁殖,进而提高了转基因小麦对全蚀病的抗性。
In order to explore the defense response of TiERF1 and RC7 to wheat against eclogioin, the molecular and total eclipse resistance of TiERF1-RC7 transgenic wheat was studied indoors and in field. The results showed that the transcribed TiERF1 and RC7 genes could be inherited and transcribed in transgenic wheat. Compared with Yangmai 18, the resistance of 5 transgenic TiERF1-RC7 wheat lines was significantly increased during the whole growth period, The total eclipse severity is less than 10%, the white spike rate is less than 13% at mature stage, while the positive rate of Yangmai 18 is 62.98% and that of white spike is 26.09%. The results of electron microscopy showed that the total mycorrhizal fungi mycelial number and growth potential in the root surface of resistant transgenic wheat were significantly lower than that of susceptible materials. The above results indicate that the introduced TiERF1 and RC7 genes inhibit the infection of all etiologic pathogens and multiply in transgenic wheat, thereby increasing the resistance of transgenic wheat to eclipse.