Surfactants, such as non-ionic Surfynol485(ethoxylated 2,4,7,9-tetramethyl-5-decyne-4,7-diol), have been applied to accelerate removal of polycyclic aromatic hydrocarbons from soil. This study investigated the dissipation of anthracene, and carbon(C) and nitrogen(N) mineralization in soil amended with non-ionic Surfynol485 at different rates. Soil samples of a Typic Fragiudept taken from Otumba, Mexico were spiked with anthracene at a final concentration of 520 mg kg-1dry soil using acetone as solvent, amended with 0.0, 24.9, 49.8 or 124.4 g kg-1soil of the surfactant and incubated in the laboratory. The soil not amended with anthracene,acetone and the surfactant was used as a control. Dynamics of C and N and the concentration of anthracene were monitored for 56 d. After 56 d of incubation, 38% of the anthracene was removed from the unamended soil, and 47%, 55% and 66% of the anthracene were removed when 24.9, 49.8 and 124.4 g kg-1of the surfactant were applied, respectively. Application of acetone, anthracene or surfactant increased the emission of CO2, but decreased the mineral N compared to the unamended control. Applying the surfactant to the acetone or anthracene-amended soil reduced emission of CO2, but increased the mineral N at the lower application rates of the surfactant. It was found that the application of the non-ionic surfactant increased the bioavailability of anthracene and thus its removal from soil, increased C mineralization, but decreased N mineralization. Consequently, the application of non-ionic surfactant could be easily used to accelerate the removal of pollutants from hydrocarbon-contaminated soils, but mineral N in the soil would decrease, which might inhibit plant growth. Surfactants, such as non-ionic Surfynol 485 (ethoxylated 2,4,7,9-tetramethyl-5-decyne-4,7-diol), have been applied to accelerate removal of polycyclic aromatic hydrocarbons from soil. anthracene, and carbon (C) and nitrogen (N) mineralization in soil amended with non-ionic Surfynol 485 at different rates. Soil samples of a Typic Fragiudept taken from Otumba, Mexico were spiked with anthracene at a final concentration of 520 mg kg- 1dry Soil using acetone as solvent, amended with 0.0, 24.9, 49.8 or 124.4 g kg-1soil of the surfactant and incubated in the laboratory. The soil not amended with anthracene, acetone and the surfactant was used as a control. Dynamics of C and N and the concentration of anthracene were monitored for 56 d. After 56 d of incubation, 38% of the anthracene was removed from the unamended soil, and 47%, 55% and 66% of the anthracene were removed when 24.9, 49.8 and 124.4 g kg-1 of the surfactant were applied, respectively. Applicati on of acetone, anthracene or surfactant increased the emission of CO2, but decreased the mineral N compared to the unamended control. Applying the surfactant to the acetone or anthracene-amended soil reduced emission of CO2, but increased the mineral N at the lower application rates of the surfactant. It was found that the application of the non-ionic surfactant increased the bioavailability of anthracene and thus its removal from soil, increased C mineralization, but decreased decreased in mineralization. Consequently, the application of non-ionic surfactant could be easily used to accelerate the removal of pollutants from hydrocarbon-contaminated soils, but mineral N in the soil would decrease, which might inhibit plant growth.