论文部分内容阅读
肿瘤生长经常伴发免疫抑制。在其成因中有源于肿瘤的免疫抑制活性物质，但其性质大多未阐明。本文报道，采用免疫组化、酶联吸附、流式细胞、核酸原位杂交等技术，观察有免疫抑制活性的逆转录病毒壳蛋白ｐ１５Ｅ在人肿瘤细胞中不同水平的表达情况。结果揭示：肿瘤细胞表达ｐ１５Ｅ比较普遍，从人食管癌、鼻咽癌、直肠癌、卵巢癌（包括癌性腹水和建株细胞系）、胃癌、乳癌等均可被检出。ＲＴ－ＰＣＲ检测显示癌患者的活化淋巴细胞ｐ１５Ｅ表达略高。这些结果提示：来自肿瘤，也许还有来自活化淋巴细胞的ｐ１５Ｅ在肿瘤伴发的免疫抑制中可能起重要作用。
Tumor growth is often accompanied by immunosuppression. In its genesis tumor-derived immunosuppressive active substances, but the nature of most of them are not elucidated. In this paper, immunohistochemistry, enzyme-linked immunosorbent assay, flow cytometry, nucleic acid in situ hybridization and other techniques were used to observe the expression of retrovirus capsid protein p15E with different levels in human tumor cells. The results revealed that the expression of p15E by tumor cells is relatively common and can be detected from human esophageal cancer, nasopharyngeal carcinoma, rectal cancer, ovarian cancer (including cancerous ascites and cell lines established), gastric cancer, breast cancer and the like. RT-PCR showed slightly higher expression of p15E in activated lymphocytes in cancer patients. These results suggest that p15E from tumors, and possibly from activated lymphocytes, may play an important role in tumor-associated immunosuppression.