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以中性红(NR)为荧光探针,利用羟丙基-β-环糊精(HP-β-CD)能够与中性红(NR)形成包合物,从而使中性红的荧光强度大大增强,而诺氟沙星的加入使NR-HP-β-CD体系的荧光猝灭,以此建立了荧光猝灭法测定诺氟沙星的新方法。在优化的实验条件下,诺氟沙星工作曲线的线性范围为:6.3×10-8～1.3×10-6mol/L,检出限为:1.9×10-8mol/L。方法可用于环境水样中痕量诺氟沙星的测定,回收率在98.4%～104.0%之间。
Neutral red (NR) as a fluorescent probe, using hydroxypropyl-β-cyclodextrin (HP-β-CD) can form an inclusion complex with the neutral red (NR), so that the neutral red fluorescence intensity Greatly enhanced, while the addition of norfloxacin quenched the fluorescence of NR-HP-β-CD system, thus establishing a new fluorescence quenching method for the determination of norfloxacin. Under the optimized experimental conditions, the linear range of norfloxacin was 6.3 × 10-8 ~ 1.3 × 10-6mol / L, and the detection limit was 1.9 × 10-8mol / L. The method can be used for the determination of trace amounts of norfloxacin in environmental water samples with recoveries between 98.4% and 104.0%.