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目的探讨虾青素对糖尿病大鼠海马脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法腹腔注射链脲佐菌素建立糖尿病大鼠模型,造模成功后,分为模型组、阳性对照组和虾青素组。阳性对照组和虾青素组分别灌胃盐酸二甲双胍和虾青素溶液。正常组灌胃等量生理盐水。药物干预6周后,分别对各组大鼠进行Morris水迷宫实验,采用real-time PCR法和ELISA法分别检测海马BDNF的表达和含量。多个样本均数比较采用单因素方差分析,两两比较采用LSD法,P<0.05为差异有统计学意义。结果四组大鼠的平台象限路径百分比和平台象限停留时间[(0.615 7±0.042)、(0.321 5±0.020)、(0.533 6±0.027)、(0.498 6±0.029)%与(43.37±6.98)、(21.46±5.65)、(36.64±5.87)、(33.18±6.35)s]比较,差异均有统计学意义(均P<0.05)。两两比较发现,与正常组相比,模型组大鼠在平台象限路径百分比和平台象限停留时间均显著减少,差异均有统计学意义(均P<0.05)。与模型组相比,阳性对照组和虾青素组在平台象限路径百分比和平台象限停留时间明显提高,差异均有统计学意义(均P<0.05)。四组大鼠BDNF含量[(5.38±0.96)、(1.72±0.33)、(3.54±0.62)、(3.17±0.57)pg/ml]比较,差异有统计学意义(P<0.05),与正常组比较,模型组BDNF的含量降低了68.03%,差异有统计学意义(P<0.05)。四组大鼠BDNF m RNA相对表达量[(0.98±0.17)、(0.53±0.10)、(0.74±0.11)、(0.75±0.12)]比较,差异有统计学意义(P<0.05),模型组BDNF的m RNA水平与正常组相比降低了45.92%,差异有统计学意义(P<0.05)。结论虾青素具有提高糖尿病大鼠认知功能的作用,其机制可能与上调BDNF的表达有关。
Objective To investigate the effects of astaxanthin on the expression of brain-derived neurotrophic factor (BDNF) in hippocampus of diabetic rats. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin. After successful modeling, the rats were divided into model group, positive control group and astaxanthin group. The positive control group and the astaxanthin group were administered with metformin hydrochloride and astaxanthin solution respectively. The normal group was given the same amount of normal saline. Six weeks after drug intervention, Morris water maze test was performed in each group, and the expression and content of BDNF in hippocampus were detected by real-time PCR and ELISA respectively. One-way analysis of variance (ANOVA) was used to compare the mean of multiple samples. LSD method was used to compare the mean of multiple samples. P <0.05 was considered statistically significant. Results The percentages of the platform quadrant pathways and the plateau quadrant retention time in the four groups [(0.615 7 ± 0.042), (0.321 5 ± 0.020), (0.533 6 ± 0.027), (0.498 6 ± 0.029)% and (43.37 ± 6.98) , (21.46 ± 5.65), (36.64 ± 5.87) and (33.18 ± 6.35) s respectively. There were significant differences between the two groups (all P <0.05). Compared with the normal group, the percentage of path of the quadrant in the model group and the dwell time of the platform quadrant in the model group were significantly decreased (all P <0.05). Compared with the model group, the percentages of the pathways of the quadrant quadrant and platform quadrant in the positive control group and the astaxanthin group increased significantly, with significant differences (all P <0.05). The BDNF content in the four groups was significantly higher than that in the normal group [(5.38 ± 0.96), (1.72 ± 0.33), (3.54 ± 0.62), (3.17 ± 0.57) pg / ml] The content of BDNF in model group decreased by 68.03%, the difference was statistically significant (P <0.05). The relative expression levels of BDNF m RNA in the four groups were significantly different ([0.98 ± 0.17], (0.53 ± 0.10), (0.74 ± 0.11), (0.75 ± 0.12)], the difference was statistically significant The m RNA level of BDNF was decreased by 45.92% compared with the normal group, the difference was statistically significant (P <0.05). Conclusion Astaxanthin can improve the cognitive function of diabetic rats and its mechanism may be related to the up-regulation of BDNF expression.