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在组织工程皮肤的体外构建中,及时观察脱细胞真皮基质(acellulardermalmatrix,ADM)表面接种的表皮细胞或成纤维细胞的形态和密度,对判断细胞存活情况、生长速度及融合程度具有重要意义。但由于真皮组织不透明,在相差显微镜下无法直接观察接种细胞的生长情况[1]。笔者拟利用组织
In vitro construction of tissue engineered skin, timely observation of the morphology and density of epidermal cells or fibroblasts inoculated on the surface of acellular dermalmatrix (ADM) is of great significance in judging cell survival, growth rate and degree of fusion. However, due to the opacity of dermal tissue, the growth of inoculated cells can not be directly observed under a phase contrast microscope [1]. I intend to use the organization