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目的:建立心律宁片中苦参碱和氧化苦参碱含量的测定方法。方法:色谱柱为Alltima Amino(250mm×4.6mm,5μm),流动相为乙腈-无水乙醇-3%磷酸溶液(80:10:10),检测波长为220nm,流速为1.0ml·min~(-1);柱温:室温。结果:苦参碱线性范围是0.168μg～3.360μg,r=0.999 7,氧化苦参碱线性范围是0.124μg～2.480μg,r=0.999 3,苦参碱及氧化苦参碱的平均回收率分别为101.1%(RSD=1.22%)和100.2%(RSD=1.91%)(n=6)。结论:方法快速简便,重复性良好,可用于测定心律宁片中苦参碱和氧化苦参碱的含量。
Objective: To establish a method for the determination of matrine and oxymatrine in Xinfuning tablets. Methods: The chromatographic column was Alltima Amino(250mm×4.6mm, 5μm). The mobile phase was acetonitrile-anhydrous ethanol-3% phosphoric acid solution (80:10:10). The detection wavelength was 220nm and the flow rate was 1.0ml·min~( -1); Column temperature: room temperature. Results: The linear range of matrine was 0.168μg～3.360μg, r=0.999 7, the linear range of oxymatrine was 0.124μg～2.480μg, r=0.999 3, the average recoveries of matrine and oxymatrine were respectively It is 101.1% (RSD=1.22%) and 100.2% (RSD=1.91%) (n=6). Conclusion: The method is rapid, simple and reproducible. It can be used to determine the content of matrine and oxymatrine in Xinfuning tablet.