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目的为评价荧光定量聚合酶链反应(FQ-PCR)在一期梅毒诊断中的临床应用价值,以暗视野(D-F)、血清学(RPR/TPHA)检查方法作对照,用FQ-PCR方法检测68例疑诊为一期梅毒病人的生殖器溃疡处分泌物。在68例疑诊为一期梅毒病人中,D-F阳性率27.94%(19/68),RPR阳性率48.53%(33/68),TPHA阳性率61.76%(42/68),FQ-PCR阳性率44.12%(30/68),FQ-PCR与D-F、TPHA比较有显著性差异(P<0.05),与RPR比较无显著性差异(P>0.1)。本研究表明FQ-PCR检测生殖器溃疡TP在一期梅毒诊断中有一定的价值。
Objective To evaluate the clinical value of fluorescent quantitative polymerase chain reaction (FQ-PCR) in the diagnosis of primary syphilis, and to compare the diagnostic methods of DF and RPR / TPHA with FQ-PCR 68 cases of suspected syphilis patients with genital ulcer secretions. The positive rate of DF was 27.94% (19/68), the positive rate of RPR was 48.53% (33/68), the positive rate of TPHA was 61.76% (42/68) in 68 cases of suspected syphilis, the positive rate of FQ-PCR 44.12% (30/68). There was a significant difference between FQ-PCR and DF and TPHA (P <0.05), but no significant difference compared with RPR (P> 0.1). This study shows that FQ-PCR detection genital ulcer TP in the diagnosis of syphilis has some value.