论文部分内容阅读
检出多环芳烃(PAH)的薄层色谱法,过去需将斑点刮下洗脱以进行最后分析,十分麻烦,为克服这种缺点,曾发表过在薄层板上的直接荧光分析法。本文即用此法,并建议予先使用柱色谱法以扩大PAH的分析范围。样品制备:样品的环己烷或苯的萃取液在室温下真空浓缩到1ml。加入一部分丙醇-2,再浓缩到1ml,如此重复2～3次,使环己烷或苯完全为丙醇-2所取代。柱色谱法:20×1cm柱,用丙醇-2湿装Sephadex LH 20,放置过夜;加入样品,以丙醇-2淋洗,分组份收集之,每组份为1.25ml。PAH的分布见附表。
Thin-layer chromatography of polycyclic aromatic hydrocarbons (PAHs) has been detected and it has been troublesome in the past to erase the spots for elution. To overcome this disadvantage, direct fluorescence analysis on thin plates has been published. This article is to use this method, and recommended the first use of column chromatography PAH to expand the scope of the analysis. Sample Preparation: The sample cyclohexane or benzene extract was concentrated in vacuo to 1 ml at room temperature. A portion of propanol-2 is added and concentrated to 1 ml. This is repeated two or three times to replace cyclohexane or benzene completely with propanol-2. Column chromatography: 20 x 1 cm column, packed with Sephadex LH 20 wet with propanol-2, left overnight; sample added, rinsed with propanol-2 and collected in fractions of 1.25 ml. PAH distribution see schedule.