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在4 mg kg~(-1)的镉污染条件下,设置不同的外源硫和硅供应水平,对辽星1号和沈农315两个品种的水稻进行了土培实验,在分蘖期对辽星1号水稻进行了亚细胞分析,观察在不同条件下重金属镉的亚细胞分布;并在分蘖期测定植株茎叶部分的非蛋白巯基(NPT)含量,研究不同硫硅配比对水稻吸收分配重金属的机理。结果表明,沈农315号水稻叶当中的NPT含量高于辽星1号,外源硫可以提升两个品种水稻叶中的NPT含量,增强对重金属的耐性,同时增加镉在包括液泡在内的细胞可溶部分的分布;施硅可以强化细胞壁,随着施硅量的提升,根茎叶细胞壁中的镉分布均显著提升,并且根细胞壁中的提升幅度大于茎,说明施硅不仅可钝化土壤中的镉,并且在植物体内,加强根细胞壁对镉的固化是硅增强植物体重金属抗性的主要途径。
Under the condition of cadmium pollution of 4 mg kg -1, different levels of exogenous sulfur and silicon were set, and two cultivars of Liaoxing 1 and Shennong 315 were cultivated in soil. During tillering stage, Liao-xing 1 was used for sub-cell analysis to observe the subcellular distribution of heavy metal cadmium under different conditions. The content of non-protein sulfhydryl (NPT) in plant stems and leaves was measured at the tillering stage. Heavy metal distribution mechanism. The results showed that the content of NPT in Shennong 315 rice leaf was higher than that in Liaoxing 1. Exogenous sulfur could increase NPT content in two varieties of rice leaf, enhance the tolerance to heavy metals, and increase the content of cadmium in vacuoles, including vacuoles The distribution of soluble part of the cell; silicon can strengthen the cell wall, with the amount of silicon increased, the distribution of cadmium in the cell wall of rhizomes significantly increased, and the increase in the root cell wall than the stem, indicating that silicon can not only passivation of soil In the plant, and in plants, to strengthen the root cell wall of the solidification of cadmium is the main way to enhance plant heavy metal resistance of plants.