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本文以放射免疫测定法测定97例正常孕妇,37例临产早期和自然分娩产妇,26例产后1～2小时妇女和10例正常非孕妇血浆免疫反应性促肾上腺皮质激素释放激素(ICRH)浓度。28例产妇在分娩同时还收集脐血,11例正常婴儿同时收集脐动脉与脐静脉血测ICRH浓度。26例产妇在收集胎盘同时采取母血。胎盘匀浆化后取上清液测ICRH浓度并行凝胶过滤。非孕、妊娠晚期、分娩、产后1、2小时妇女血样及脐血以促肾上腺皮质激素释放激素(CRH)抗血清免疫亲和层析法提取CRH,再将洗脱液以SephadexG75行凝胶过滤。分娩时母血
In this paper, 97 cases of normal pregnant women, 37 cases of early labor and natural childbirth, 26 cases of postpartum 1 to 2 hours and 10 normal non-pregnant women plasma immunoreactive corticotropin-releasing hormone (ICRH) concentrations were measured by radioimmunoassay. 28 maternal women also collected umbilical cord blood during delivery, and 11 normal infants also collected ICRH concentrations measured by umbilical artery and umbilical vein at the same time. Twenty-six women took maternal blood while collecting the placenta. After homogenization of the placenta, the supernatant was taken to measure ICRH concentration and subjected to gel filtration. Non-pregnant, late pregnancy, childbirth, 1 hour and 2 hours postpartum women’s blood samples and umbilical cord blood with corticotropin-releasing hormone (CRH) antiserum immunoaffinity chromatography extraction of CRH, and then the eluate gel filtration SephadexG75 line . Maternal blood during childbirth