论文部分内容阅读
目的探讨妊娠期高血压胎盘组织中TGF-β1及Smad的表达及意义。方法应用RT-PCR方法及免疫组化法检测胎盘组织TGF-β1及Smad的表达。结果 RT-PCR结果显示:子痫前期胎盘组织中TGF-β1及Smad mRNA的表达量均增加。重度子痫前期胎盘组织中TGF-β1及Smad mRNA的表达量与正常妊娠组相比有统计学差异(P<0.01),轻度子痫前期组中的表达与正常妊娠组间有统计学差异(P<0.05)。免疫组化法显示胎盘组织中TGF-β1及Smad阳性着色部位均位于胞浆,细胞定位于绒毛小叶的蜕膜细胞、合体滋养细胞和细胞滋养细胞。TGF-β1及Smad积分光密度值在妊娠期高血压胎盘中比正常组胎盘中增高明显,具有显著差异,有统计学意义(P<0.05)。结论胎盘组织中TGF-β1及Smad的表达升高与妊娠期高血压疾病的发病有关。
Objective To investigate the expression and significance of TGF-β1 and Smad in placenta of hypertensive rats during pregnancy. Methods The expression of TGF-β1 and Smad in placenta were detected by RT-PCR and immunohistochemistry. Results RT-PCR results showed that the expression of TGF-β1 and Smad mRNA in preeclampsia placenta increased. The expression of TGF-β1 and Smad mRNA in the severe preeclampsia placenta was significantly higher than that in the normal pregnancy group (P <0.01). The expression in the mild preeclampsia group was significantly different from that in the normal pregnancy group (P <0.05). Immunohistochemistry showed that placental TGF-β1 and Smad-positive staining sites were located in the cytoplasm, the cells located in the villous leaf decidual cells, syncytiotrophoblasts and cytotrophoblasts. The integral optical density of TGF-β1 and Smad increased significantly in the placenta of gestational hypertension than in the normal group, with significant difference (P <0.05). Conclusion The increased expression of TGF-β1 and Smad in placenta is related to the incidence of hypertensive disorders in pregnancy.