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目的观察SKI-Ⅱ对胃癌SGC7901细胞内血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,并对其机制进行初步研究。方法常规培养胃癌SGC7901细胞,给予不同浓度SKI-Ⅱ及顺铂(DDP)干预,MTT法测定各组胃癌SGC7901细胞增殖情况,免疫组化和Western blot检测药物对SphK1、VEGF表达的影响。结果 MTT检测显示SGC7901细胞与不同浓度的SKI-Ⅱ共同培养,细胞的生长受到不同程度的抑制,其作用具有剂量依赖性,并随时间的延长逐渐增强,免疫组化及Western blot检测显示SKI-Ⅱ可显著下调肿瘤细胞SphK1和VEGF蛋白的表达,单用DDP对SphK1、VEGF无作用。结论 SKI-Ⅱ可以通过下调VEGF和SphK1蛋白表达而有效抑制胃癌SGC7901细胞增殖。
Objective To investigate the effect of SKI-Ⅱ on the expression of vascular endothelial growth factor (VEGF) in gastric cancer cell line SGC7901 and its mechanism. Methods The gastric cancer SGC7901 cells were cultured routinely. The cells were treated with different concentrations of SKI-Ⅱ and DDP. The proliferation of SGC7901 cells was determined by MTT assay. The expressions of SphK1 and VEGF were detected by immunohistochemistry and Western blot. Results MTT assay showed that SGC7901 cells were co-cultured with different concentrations of SKI-Ⅱ. The growth of SGC7901 cells was inhibited to a certain extent. The effect of SGC7901 cells was dose-dependent and gradually increased with time. Immunohistochemistry and Western blot showed that SKI- Ⅱ could significantly down-regulate the expression of SphK1 and VEGF protein in tumor cells. DDP alone had no effect on SphK1 and VEGF. Conclusion SKI-Ⅱ can effectively inhibit the proliferation of gastric cancer SGC7901 cells by downregulating the expression of VEGF and SphK1.