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为了探讨人早孕滋养层组织中人绒毛膜促性腺激素（ｈＣＧ）分泌的自分泌和旁分泌调节机制。将人工流产获得的正常绒毛组织剪成１ｍｍ３小块接种在涂有胶原的培养瓶中，每瓶加入２ｍｌ含有１．７５ｎｍｏｌ／ＬＮ－２－羟乙基哌嗪－Ｎ＇－２乙烷磺酸（ＨＥＰＥＳ）、２ｎｍｏｌ／Ｌ谷氨酰胺、１０５ＩＵ／Ｌ青霉素和１００ｍｇ／Ｌ链霉素的ＭｃＣｏｙ５ａ培养液，绒毛组织先培养在３７℃的ＣＯ２孵箱中（ＣＯ２：空气＝５％：９５％）４８ｈ，以排除其内源的激素，然后加入各种激素或作用物，每天采集并更换一次培养液，收集的培养液存于－２０℃留待放射免疫测定用。实验结束时以１ｍｏｌ／ＬＮａＯＨ裂解组织块供蛋白质测定用。结果表明ＧｎＲＨ对ｈＣＧ分泌呈现双相的作用：低剂量刺激而高剂量抑制ｈＣＧ分泌，而ＴＲＨ对滋养层未见效应。去甲肾上腺素亦具有促进ｈＣＧ分泌的作用，并呈现剂量依赖反应，随去甲肾上腺素处理时间的增长，其刺激效应亦更为明显。阻断内源孕酮的转化和代谢，或加入外源孕酮或其前体，均不影响ｈＣＧ的分泌。此外，睾酮、雌二醇、前列腺素Ｅ２（ＰＧＥ２）和前列腺素Ｆ２α（ＰＧＦ２α）等激素对ｈＣＧ的分泌也无直接影响。研究结果进一步证实了胎盘内存在激素分泌的
In order to investigate the mechanism of autocrine and paracrine regulation of human chorionic gonadotropin (hCG) secretion in human trophoblast in early pregnancy. The normal villi obtained by artificial abortion were cut into small pieces of 1 mm3 and inoculated in collagen coated flasks, and 2 ml of a solution containing 1.75 nmol / LN-2-hydroxyethylpiperazine-N’-2 ethanesulfonic acid (HEPES), 2nmol / L glutamine, 105IU / L penicillin and 100mg / L streptomycin. The villous tissue was cultured in a CO2 incubator at 37 ℃ (CO2: air = 5%: 95% 48h, to exclude the endogenous hormones, and then add a variety of hormones or agents, daily culture and replacement of a medium, collected culture medium stored at -20 ℃ for radioimmunoassay. Tissue pieces were lysed with 1 mol / L NaOH for protein determination at the end of the experiment. The results showed that GnRH exhibited a biphasic effect on hCG secretion: low dose stimulation and high dose inhibition of hCG secretion, while TRH had no effect on trophoblast. Norepinephrine also has a role in promoting hCG secretion, and showed a dose-dependent response, with the norepinephrine treatment time increases, the stimulus effect is also more pronounced. Blocking the conversion and metabolism of endogenous progesterone or adding exogenous progesterone or its precursor did not affect the secretion of hCG. In addition, hormones such as testosterone, estradiol, prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) have no direct effect on hCG secretion. The results further confirmed the presence of hormone secretion in the placenta