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目的探讨测定胃癌患者外周血单个核细胞(PBMC)及肿瘤组织细胞表面分化抗原44变异体6(CD44v6)含量(%)的临床意义。方法用流式细胞术(FCM)检测50例胃癌患者术前和术后1周及20名正常人PBMCCD44v6含量(%),以及术后胃癌及癌旁正常组织CD44v6含量。结果胃癌患者术前PBMCCD44v6含量(9.72%±6.65%)比正常对照组(3.11%±1.15%)及术后1周(4.44%±2.33%)明显增高(分别P<0.001和<0.01);胃癌及癌旁正常组织CD44v6含量分别为3.67%±0.63%及1.33%±0.51%(P<0.01);Ⅲ+Ⅳ期胃癌患者PBMC及胃癌组织CD44v6含量(分别为12.56%±6.97%及3.91%±0.87%)均明显高于Ⅰ+Ⅱ期患者(分别为6.89%±3.37%和2.86%±0.65%,均P<0.05)。胃癌淋巴转移患者PBMC及胃癌组织CD44v6含量(分别为11.29%±5.36%和4.41%±0.79%)均明显高于无转移者(7.32%±4.28%和2.61%±0.78%,均P<0.05)。结论用FCM检测胃癌患者PBMCCD44v6含量能反映胃癌组织CD44v6改变,对胃癌诊断、进展与转移评价及术后疗效观察具有重要的临床意义。
Objective To investigate the clinical significance of determining the content (%) of peripheral blood mononuclear cells (PBMCs) and the differentiation of tumor cell surface differentiation antigen 44 (CD44v6) in patients with gastric cancer. Methods Flow cytometry (FCM) was used to detect the percentage of CD44v6 (%) in preoperative and postoperative 1 week and 20 normal PBMCs of 50 patients with gastric cancer and the expression of CD44v6 in gastric cancer tissues and adjacent normal tissues. Results The preoperative PBMCCD44v6 content in gastric cancer patients was significantly higher than that in the normal control group (3.11% ± 1.15% vs 4.44% ± 2.33%, P <0.001 and <0.01, respectively) (9.72% ± 6.65% (CD44v6) and CD44v6 (3.33% ± 0.63%, 1.33% ± 0.51%, respectively) in normal gastric mucosa tissues and adjacent normal tissues (P <0.01) 0.87%) were significantly higher than those in stage I + II patients (6.89% ± 3.37% and 2.86% ± 0.65%, respectively, P <0.05). The levels of CD44v6 in PBMCs and gastric cancer tissues from patients with lymph node metastasis (11.29% ± 5.36% and 4.41% ± 0.79%, respectively) were significantly higher than those without metastasis (7.32% ± 4.28% and 2.61% ± 0.78%, both P <0.05) . Conclusion The detection of CD44v6 in PBMCs by FCM can reflect the change of CD44v6 in gastric cancer tissues. It has important clinical significance in the diagnosis, progression and metastasis of gastric cancer and the observation of postoperative efficacy.