13.1 Pancreatitis 2006384 Changes of endothelin levels and therapeutic effects of Danshen in lung injury induced by severe acute pancreatitis in rats. HUANG Xiao-li, et al. Dept of Gastroenterol, Nanjing Jiang-bei Hosp Nanjing 210048. World Chin J Dig 2006;14(9):853-857. Objective:To explore the changes of endothelin (ET) levels in serum and lung, and the therapeutic effects of labiatae on severe acute pancreatitis (SAP) with lung injury in rats. Methods:A total of 60 Wistar rats were randomized into sham operation group (J), model group (F), and Danshen treatment group (D). The model of SAP was established by retrograde injection of 50 g/L sodium taurocholate into the pancreatic and biliary duct. The rats in group D were intraperitoneally injected with Danshen (5 mL/kg) 1 d before and 10 min after modeling. The levels of serum and pulmonary endothelin-1 were evaluated 24 and 48 h after modeling. Meanwhile, the changes of pulmonary histopathology and lung index were observed. Results:In comparison with those in group J, the pulmonary histopathological changes in group F were significantly aggravated, and the level of serum endothelin-1 and lung index were increased markedly 24 and 48 h after modeling (endothelin-1: 75.8±4.8, 70.4±4.8 ng/L vs 32.0±6.9, 30.3±4.8 ng/L, P<0.01; lung index: 0.62±0.06, 0.73±0.07 vs 0.41±0.08, 0.41±0.07, P<0.01). Meanwhile, the expression of pulmonary endothelin-1 (using optical density) was elevated (F group: 0.48±0.09, 0.61±0.10; J group: 0.05±0.01, 0.05±0.01, P<0.01; for 24 and 48 h respectively). Compared with those in group F, the pulmonary pathological changes in group D were notably alleviated, and the levels of serum endothelin-1 and lung index were notably dropped 24 and 48 h after modeling (60.2±7.3 ng/L, 0.52±0.06, P<0.05; 57.9±5.4 ng/L, 0.58±0.06, P<0.01), and the expression of pulmonary endothelin-1 was also decreased significantly (0.23±0.10, 0.36±0.09, for 24 and 48 h, respectively, P<0.01 vs group F). 13.1 Pancreatitis 2006 384 Changes of endothelin levels and therapeutic effects of Danshen in lung injury induced by severe acute pancreatitis in rats. HUANG Xiao-li, et al. Dept of Gastroenterol, Nanjing Jiang-bei Hosp Nanjing 210048. World Chin J Dig 2006; 14 (9): 853-857. Objective: To explore the changes of endothelin (ET) levels in serum and lung, and the therapeutic effects of labiatae on severe acute pancreatitis (SAP) with lung injury in rats. Methods: A total of 60 Wistar rats were randomized into sham operation group (J), model group (F), and Danshen treatment group (D). The model of SAP was established by retrograde injection of 50 g / L sodium taurocholate into the pancreatic and biliary duct. rats in group D were intraperitoneally injected with Danshen (5 mL / kg) for 1 d before and 10 min after modeling. The levels of serum and pulmonary endothelin-1 were evaluated 24 and 48 h after modeling. Meanwhile, the changes of pulmonary histopathology and lung index were observed n comparison with those in group J, the pulmonary histopathological changes in group F were significantly aggravated, and the level of serum endothelin-1 and lung index were significantly markedly 24 and 48 h after modeling (endothelin-1: 75.8 ± 4.8, 70.4 ± (P <0.01), 4.8 ng / L vs 32.0 ± 6.9, 30.3 ± 4.8 ng / L, P <0.01; lung index: 0.62 ± 0.06, 0.73 ± 0.07 vs 0.41 ± 0.08, Compared with those in group (F group: 0.48 ± 0.09, 0.61 ± 0.10; J group: 0.05 ± 0.01, 0.05 ± 0.01, P <0.01; for 24 and 48 h respectively) F, the pulmonary pathological changes in group D were notably alleviated, and the levels of serum endothelin-1 and lung index were not otherwise dropped 24 and 48 h after modeling (60.2 ± 7.3 ng / L, 0.52 ± 0.06, P <0.05; 57.9 ± 5.4 ng / L, 0.58 ± 0.06, P <0.01), and the expression of pulmonary endothelin-1 was also also significantly (0.23 ± 0.10, 0.36 ± 0.09, for 24 and 48 h, respectively, P <0.01 vs. group F ).