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本实验以二甲基氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌的动物模型为材料，用电脉方法观察了在诱癌过程中和肝癌形成后大白鼠肝细胞质膜上蛋白质的变化。肝细胞质膜蛋白的ＳＤＳ—ＰＡＧＥ电泳图谱显示：在诱癌４周、１６周的肝细胞和肝细胞癌的细胞质膜蛋白与正常肝比较没有显著差异。胆管癌细胞质膜蛋白的ＳＤＳ—ＰＡＧＥ电泳图谱与正常肝细胞比较，在１５．８ＫＤ～６９．２ＫＤ的带群中新出现一条深染的蛋白带。用双向电泳方法对肝细胞质膜蛋白进行分析，诱癌４周和１６周的肝细胞质膜蛋白双向电泳图谱与正常肝比较，除了等电点低于ｐＨ６．８４的蛋白质点数略有增加外，没有显著差异。在肝细胞癌质膜蛋白双向电泳图谱上，等电点高于ｐＨ７．８３的蛋白质点数略有减少，等电点低于ｐＨ６．８４的蛋白质点数显著增加
In this experiment, animal model of hepatocarcinogenesis induced by DAB in rats was used as material. The changes of protein in the plasma membrane of liver cells were observed by electrocardiogram in the process of carcinogenesis and hepatocarcinogenesis. SDS-PAGE of hepatocyte plasma membrane proteins showed no significant difference in the plasma membrane proteins of hepatocytes and hepatocellular carcinoma at 16 weeks after induction of cancer compared with normal liver. Compared with normal hepatocytes, SDS-PAGE of plasma membrane proteins of cholangiocarcinoma cells showed a newly stained protein band in the band of 15.8KD to 69.2KD. By two-dimensional electrophoresis of liver cell plasma membrane protein analysis, induced cancer 4 weeks and 16 weeks liver cell plasma membrane two-dimensional electrophoresis compared with the normal liver, in addition to the isoelectric point below pH6.84 protein points slightly increased, there is no Significant differences. In the two-dimensional gel electrophoresis map of hepatoma cell membrane protein, the number of spots with isoelectric point higher than that of pH7.83 slightly decreased, and the number of spots with isoelectric point lower than pH6.84 increased significantly