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目的:通过体内模型研探讨七叶皂苷钠对H22小鼠肝癌移植瘤的抗肿瘤作用机制。方法:采用ICR小鼠H22荷瘤肝癌模型观察七叶皂苷钠抗肿瘤作用,采用Western Blot分析肿瘤组织中相关蛋白的表达变化,通过免疫组化分析肿瘤组织中CD31变化,评价微血管密度。结果:1.4和2.8 mg.kg-1七叶皂苷钠对H22的抑瘤率分别为19.2%,40.7%。免疫组化结果显示,七叶皂苷钠能够显著降低肿瘤内部微血管密度,低高剂量组可分别达到44.1%和48.5%。Western Blot证实,七叶皂苷钠能不同程度下调cyclinD1、cdk2、cyclinE、VEGF、p-Akt等蛋白的表达,抑制p65的核转位。结论:七叶皂苷钠有一定的抗肿瘤作用,其机制可能与阻滞细胞周期,抑制血管新生以及干扰信号转导有关。
OBJECTIVE: To investigate the antitumor mechanism of sodium aescinate on hepatocellular carcinoma xenografts in H22 mice in vivo. Methods: The antitumor effect of sodium aescinate was observed by H22 tumor-bearing liver cancer model in ICR mice. Western Blot was used to analyze the expression of related proteins in tumor tissues. The CD31 changes in tumor tissues were analyzed by immunohistochemistry to evaluate the microvessel density. Results: The inhibitory rates of sodium aescinate 1.4 and 2.8 mg.kg-1 to H22 were 19.2% and 40.7%, respectively. The results of immunohistochemistry showed that sodium aescinate significantly reduced the density of microvessel inside the tumor, reaching 44.1% and 48.5% respectively in the low-dose group. Western Blot confirmed that sodium aescinate can down-regulate the expression of cyclinD1, cdk2, cyclinE, VEGF, p-Akt and other proteins, inhibiting the nuclear translocation of p65. CONCLUSION: Sodium aescinate has certain anti-tumor effect, and its mechanism may be related to arresting cell cycle, inhibiting angiogenesis and interfering with signal transduction.